CRISPR

CRISPR2-hspa8

ID
ZDB-CRISPR-240102-3
Name
CRISPR2-hspa8
Previous Names
None
Target
Sequence
5' - GGAAATTGCTGAGGCCTACC - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
None
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
ouc8 hspa8
ouc9 hspa8
Expression
Gene expression in Wild Types + CRISPR2-hspa8
No data available
Phenotype
Phenotype resulting from CRISPR2-hspa8
No data available
Phenotype of all Fish created by or utilizing CRISPR2-hspa8
Phenotype Fish Conditions Figures
pharyngeal arch cartilage atf4b expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
pharyngeal arch cartilage Ab3-eif2a labeling increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
pharyngeal arch cartilage ccng1 expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 4 from Wang et al., 2022
pharyngeal arch cartilage dlx2a expression decreased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 2 from Wang et al., 2022
pharyngeal arch cartilage hsp90aa1.1 expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
whole organism ab11-tp53 labeling increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 4 from Wang et al., 2022
pharyngeal arch cartilage tp53 expression amount, ameliorated hspa8ouc8/ouc8 chemical treatment by environment: ISRIB Fig. 8 from Wang et al., 2022
eye decreased size, abnormal hspa8ouc8/ouc8 standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage tp53 expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 4,  Fig. 8 from Wang et al., 2022
pectoral fin decreased size, abnormal hspa8ouc8/ouc8 standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage cdkn1a expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 4 from Wang et al., 2022
pharyngeal arch cartilage malformed, abnormal hspa8ouc8/ouc8 standard conditions Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage atf5a expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
pharyngeal arch cartilage atf6 expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
pharyngeal arch cartilage serpinh1b expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
pharyngeal arch cartilage trib3 expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
pharyngeal arch cartilage cdkn1bb expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 4 from Wang et al., 2022
whole organism hspa8 expression decreased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 1 from Wang et al., 2022
head Ab2-hspa8 labeling decreased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage Ab1-atf4 labeling increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7,  Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage decreased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage bbc3 expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 4 from Wang et al., 2022
head decreased size, abnormal hspa8ouc8/ouc8 standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch IRE1-mediated unfolded protein response increased occurrence, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
splanchnocranium malformed, abnormal hspa8ouc8/ouc8 standard conditions Fig. 1 from Wang et al., 2022
head ab11-tp53 labeling increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 5 from Wang et al., 2022
pericardium edematous, abnormal hspa8ouc8/ouc8 standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cellular response to unfolded protein increased occurrence, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
pharyngeal arch cartilage Ab1-atf4 labeling amount, ameliorated hspa8ouc8/ouc8 chemical treatment by environment: ISRIB Fig. 8 from Wang et al., 2022
whole organism ccng1 expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 4 from Wang et al., 2022
whole organism cdkn1a expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 4 from Wang et al., 2022
whole organism tp53 expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 4 from Wang et al., 2022
pharyngeal arch regulation of signal transduction by p53 class mediator increased occurrence, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
pharyngeal arch cartilage ddit3 expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
whole organism Ab2-hspa8 labeling decreased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 1,  Fig. 4 from Wang et al., 2022
pharyngeal arch cartilage sox9a expression decreased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 2 from Wang et al., 2022
pharyngeal arch cartilage atf3 expression increased amount, abnormal hspa8ouc8/ouc8 standard conditions Fig. 7 from Wang et al., 2022
pharyngeal arch cartilage malformed, ameliorated hspa8ouc8/ouc8 chemical treatment by environment: ISRIB Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage Ab3-eif2a labeling increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 7 from Wang et al., 2022
pharyngeal arch cartilage ccng1 expression increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 4 from Wang et al., 2022
eye decreased size, abnormal hspa8ouc9/ouc9 standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage dlx2a expression decreased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 2 from Wang et al., 2022
whole organism ab11-tp53 labeling increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 4 from Wang et al., 2022
pharyngeal arch cartilage tp53 expression amount, ameliorated hspa8ouc9/ouc9 chemical treatment by environment: ISRIB Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage cdkn1a expression increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 4 from Wang et al., 2022
pharyngeal arch cartilage tp53 expression increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 4,  Fig. 8 from Wang et al., 2022
pectoral fin decreased size, abnormal hspa8ouc9/ouc9 standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage malformed, abnormal hspa8ouc9/ouc9 standard conditions Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage cdkn1bb expression increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 4 from Wang et al., 2022
whole organism hspa8 expression decreased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 1 from Wang et al., 2022
head Ab2-hspa8 labeling decreased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage Ab1-atf4 labeling increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 7,  Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage bbc3 expression increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 4 from Wang et al., 2022
head decreased size, abnormal hspa8ouc9/ouc9 standard conditions Fig. 1 from Wang et al., 2022
splanchnocranium malformed, abnormal hspa8ouc9/ouc9 standard conditions Fig. 1 from Wang et al., 2022
head ab11-tp53 labeling increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 5 from Wang et al., 2022
pericardium edematous, abnormal hspa8ouc9/ouc9 standard conditions Fig. 1 from Wang et al., 2022
whole organism tp53 expression increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 4 from Wang et al., 2022
whole organism ccng1 expression increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 4 from Wang et al., 2022
whole organism cdkn1a expression increased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 4 from Wang et al., 2022
pharyngeal arch cartilage Ab1-atf4 labeling amount, ameliorated hspa8ouc9/ouc9 chemical treatment by environment: ISRIB Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage sox9a expression decreased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 2 from Wang et al., 2022
whole organism Ab2-hspa8 labeling decreased amount, abnormal hspa8ouc9/ouc9 standard conditions Fig. 1,  Fig. 4 from Wang et al., 2022
pharyngeal arch cartilage malformed, ameliorated hspa8ouc9/ouc9 chemical treatment by environment: ISRIB Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage EGFP expression spatial pattern, ameliorated hspa8ouc8/ouc8; y1Tg/y1Tg chemical treatment by environment: ISRIB Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage EGFP expression decreased distribution, abnormal hspa8ouc8/ouc8; y1Tg/y1Tg standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage ab1-col2a labeling spatial pattern, abnormal hspa8ouc8/ouc8; y1Tg/y1Tg standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage ab1-col2a labeling decreased amount, abnormal hspa8ouc8/ouc8; y1Tg/y1Tg standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage EGFP expression spatial pattern, abnormal hspa8ouc8/ouc8; y1Tg/y1Tg standard conditions Fig. 1,  Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage cell division decreased frequency, abnormal hspa8ouc8/ouc8; y1Tg/y1Tg standard conditions Fig. 3 from Wang et al., 2022
head decreased size, abnormal hspa8ouc9/+; hspa8ouc8/ouc8; hspa8ouc7/+ standard conditions Fig. 1 from Wang et al., 2022
pericardium edematous, abnormal hspa8ouc9/+; hspa8ouc8/ouc8; hspa8ouc7/+ standard conditions Fig. 1 from Wang et al., 2022
eye decreased size, abnormal hspa8ouc9/+; hspa8ouc8/ouc8; hspa8ouc7/+ standard conditions Fig. 1 from Wang et al., 2022
splanchnocranium malformed, abnormal hspa8ouc9/+; hspa8ouc8/ouc8; hspa8ouc7/+ standard conditions Fig. 1 from Wang et al., 2022
pectoral fin decreased size, abnormal hspa8ouc9/+; hspa8ouc8/ouc8; hspa8ouc7/+ standard conditions Fig. 1 from Wang et al., 2022
head decreased size, abnormal hspa8ouc9/ouc9; hspa8ouc7/ouc7 standard conditions Fig. 1 from Wang et al., 2022
eye decreased size, abnormal hspa8ouc9/ouc9; hspa8ouc7/ouc7 standard conditions Fig. 1 from Wang et al., 2022
pericardium edematous, abnormal hspa8ouc9/ouc9; hspa8ouc7/ouc7 standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage decreased amount, abnormal hspa8ouc9/ouc9; hspa8ouc7/ouc7 standard conditions Fig. 1 from Wang et al., 2022
splanchnocranium malformed, abnormal hspa8ouc9/ouc9; hspa8ouc7/ouc7 standard conditions Fig. 1 from Wang et al., 2022
pectoral fin decreased size, abnormal hspa8ouc9/ouc9; hspa8ouc7/ouc7 standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage EGFP expression spatial pattern, ameliorated hspa8ouc9/ouc9; y1Tg/y1Tg chemical treatment by environment: ISRIB Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage EGFP expression decreased distribution, abnormal hspa8ouc9/ouc9; y1Tg/y1Tg standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage ab1-col2a labeling spatial pattern, abnormal hspa8ouc9/ouc9; y1Tg/y1Tg standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage ab1-col2a labeling decreased amount, abnormal hspa8ouc9/ouc9; y1Tg/y1Tg standard conditions Fig. 1 from Wang et al., 2022
pharyngeal arch cartilage EGFP expression spatial pattern, abnormal hspa8ouc9/ouc9; y1Tg/y1Tg standard conditions Fig. 1,  Fig. 8 from Wang et al., 2022
pharyngeal arch cartilage cell division decreased frequency, abnormal hspa8ouc9/ouc9; y1Tg/y1Tg standard conditions Fig. 3 from Wang et al., 2022
pharyngeal arch cartilage decreased amount, abnormal tp53zdf1/+; hspa8ouc8/ouc8 standard conditions Fig. 5 from Wang et al., 2022
eye decreased size, abnormal tp53zdf1/+; hspa8ouc8/ouc8 standard conditions Fig. 5 from Wang et al., 2022
whole organism tp53 expression increased amount, abnormal tp53zdf1/+; hspa8ouc8/ouc8 standard conditions Fig. 6 from Wang et al., 2022
whole organism ab11-tp53 labeling increased amount, abnormal tp53zdf1/+; hspa8ouc8/ouc8 standard conditions Fig. 6 from Wang et al., 2022
whole organism ccng1 expression increased amount, abnormal tp53zdf1/+; hspa8ouc8/ouc8 standard conditions Fig. 6 from Wang et al., 2022
pectoral fin decreased size, abnormal tp53zdf1/+; hspa8ouc8/ouc8 standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage sox9a expression spatial pattern, abnormal tp53zdf1/+; hspa8ouc8/ouc8; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage EGFP expression spatial pattern, abnormal tp53zdf1/+; hspa8ouc8/ouc8; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage sox9a expression decreased amount, abnormal tp53zdf1/+; hspa8ouc8/ouc8; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage decreased amount, abnormal tp53zdf1/+; hspa8ouc9/ouc9 standard conditions Fig. 5 from Wang et al., 2022
eye decreased size, abnormal tp53zdf1/+; hspa8ouc9/ouc9 standard conditions Fig. 5 from Wang et al., 2022
whole organism tp53 expression increased amount, abnormal tp53zdf1/+; hspa8ouc9/ouc9 standard conditions Fig. 6 from Wang et al., 2022
whole organism ab11-tp53 labeling increased amount, abnormal tp53zdf1/+; hspa8ouc9/ouc9 standard conditions Fig. 6 from Wang et al., 2022
pectoral fin decreased size, abnormal tp53zdf1/+; hspa8ouc9/ouc9 standard conditions Fig. 5 from Wang et al., 2022
whole organism ccng1 expression increased amount, abnormal tp53zdf1/+; hspa8ouc9/ouc9 standard conditions Fig. 6 from Wang et al., 2022
pharyngeal arch cartilage sox9a expression spatial pattern, abnormal tp53zdf1/+; hspa8ouc9/ouc9; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage EGFP expression spatial pattern, abnormal tp53zdf1/+; hspa8ouc9/ouc9; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage sox9a expression decreased amount, abnormal tp53zdf1/+; hspa8ouc9/ouc9; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
whole organism ccng1 expression amount, ameliorated tp53zdf1/zdf1; hspa8ouc8/ouc8 standard conditions Fig. 6 from Wang et al., 2022
whole organism tp53 expression amount, ameliorated tp53zdf1/zdf1; hspa8ouc8/ouc8 standard conditions Fig. 6 from Wang et al., 2022
pectoral fin decreased size, ameliorated tp53zdf1/zdf1; hspa8ouc8/ouc8 standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage decreased amount, abnormal tp53zdf1/zdf1; hspa8ouc8/ouc8 standard conditions Fig. 5 from Wang et al., 2022
whole organism ab11-tp53 labeling increased amount, abnormal tp53zdf1/zdf1; hspa8ouc8/ouc8 standard conditions Fig. 6 from Wang et al., 2022
eye decreased size, ameliorated tp53zdf1/zdf1; hspa8ouc8/ouc8 standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage sox9a expression spatial pattern, abnormal tp53zdf1/zdf1; hspa8ouc8/ouc8; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage sox9a expression decreased amount, abnormal tp53zdf1/zdf1; hspa8ouc8/ouc8; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage EGFP expression spatial pattern, abnormal tp53zdf1/zdf1; hspa8ouc8/ouc8; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
whole organism ccng1 expression amount, ameliorated tp53zdf1/zdf1; hspa8ouc9/ouc9 standard conditions Fig. 6 from Wang et al., 2022
whole organism tp53 expression amount, ameliorated tp53zdf1/zdf1; hspa8ouc9/ouc9 standard conditions Fig. 6 from Wang et al., 2022
pectoral fin decreased size, ameliorated tp53zdf1/zdf1; hspa8ouc9/ouc9 standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage decreased amount, abnormal tp53zdf1/zdf1; hspa8ouc9/ouc9 standard conditions Fig. 5 from Wang et al., 2022
whole organism ab11-tp53 labeling increased amount, abnormal tp53zdf1/zdf1; hspa8ouc9/ouc9 standard conditions Fig. 6 from Wang et al., 2022
eye decreased size, ameliorated tp53zdf1/zdf1; hspa8ouc9/ouc9 standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage sox9a expression spatial pattern, abnormal tp53zdf1/zdf1; hspa8ouc9/ouc9; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage sox9a expression decreased amount, abnormal tp53zdf1/zdf1; hspa8ouc9/ouc9; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
pharyngeal arch cartilage EGFP expression spatial pattern, abnormal tp53zdf1/zdf1; hspa8ouc9/ouc9; y1Tg/y1Tg standard conditions Fig. 5 from Wang et al., 2022
Citations
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