CRISPR

CRISPR1-rbfox1l

ID

ZDB-CRISPR-231201-4

Name

CRISPR1-rbfox1l

Previous Names

None

Target

rbfox1l (1)

Sequence

5' - TGTGGCAGCGGCGGCTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Genome Build: GRCz11Chromosome: 16

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
chb5	rbfox1l

Expression

Gene expression in Wild Types + CRISPR1-rbfox1l

No data available

Phenotype

Phenotype resulting from CRISPR1-rbfox1l

No data available

Phenotype of all Fish created by or utilizing CRISPR1-rbfox1l

Phenotype	Fish	Conditions	Figures
positive regulation of RNA splicing decreased process quality, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 4 from Huang et al., 2022
bulbus arteriosus ab3-tnnt labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 6 from Huang et al., 2022
dorsal aorta blood vessel endothelium obstructed, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 6 from Huang et al., 2022
mitochondrial gene expression decreased rate, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 4 from Huang et al., 2022
cardiac muscle cell positive regulation of oxygen metabolic process decreased process quality, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
cardiac muscle cell mitochondrion decreased size, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
bulbus arteriosus ab1-elnb labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 6 from Huang et al., 2022
pericardium edematous, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 6 from Huang et al., 2022
cardiac muscle cell sarcomere ab-mf20 labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
sarcomere organization decreased process quality, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 4 from Huang et al., 2022
cardiac muscle cell Z disc ab3-tnnt labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
heart contraction decreased process quality, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 6 from Huang et al., 2022
cardiac muscle cell sarcomere ab-s46 labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
cardiac muscle cell ATP metabolic process decreased process quality, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
cardiac muscle cell sarcomere ab3-tnnt labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
cardiac muscle cell mitochondrion increased amount, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
endocardial cushion zn-8 labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 6 from Huang et al., 2022
cardiac muscle cell mitochondrial crista disorganized, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
cardiac muscle cell NAD biosynthetic process decreased process quality, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
cardiac muscle cell Z disc ab-mf20 labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
cardiac muscle cell Z disc ab-s46 labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6	standard conditions	Fig. 5 from Huang et al., 2022
cardiac muscle cell EGFP expression spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 2 from Huang et al., 2022
cardiac muscle cell zn-8 labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 2 from Huang et al., 2022
bulbus arteriosus ab1-elnb labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 2 from Huang et al., 2022
cardiac muscle cell EGFP expression decreased amount, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 2 from Huang et al., 2022
bulbus arteriosus elongated, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 2 from Huang et al., 2022
whole organism paralysed, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 1 from Huang et al., 2022
pericardium edematous, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 1 from Huang et al., 2022
heart contraction decreased process quality, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 1 , Fig. 3 from Huang et al., 2022
cardiac ventricle cardiac muscle cell decreased area, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 3 from Huang et al., 2022
blood circulation decreased process quality, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 1 from Huang et al., 2022
whole organism dead, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 1 from Huang et al., 2022
bulbus arteriosus decreased width, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 2 from Huang et al., 2022
cardiac ventricle cardiac muscle cell zn-8 labeling spatial pattern, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; f1Tg/f1Tg	standard conditions	Fig. 3 from Huang et al., 2022
cardiac muscle cell decreased size, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; fb18Tg/fb18Tg	standard conditions	Fig. 3 from Huang et al., 2022
cardiac ventricle cell division decreased frequency, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; fb18Tg/fb18Tg	standard conditions	Fig. 2 from Huang et al., 2022
endocardial cushion absent, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; s896Tg/s896Tg	standard conditions	Fig. 2 from Huang et al., 2022
dorsal aorta blood vessel endothelium obstructed, abnormal	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; s896Tg/s896Tg	standard conditions	Fig. 2 from Huang et al., 2022
dorsal aorta blood vessel endothelium normal object quality, ameliorated	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; chb7Tg/chb7Tg	standard conditions	Fig. 6 from Huang et al., 2022
bulbus arteriosus ab1-elnb labeling spatial pattern, ameliorated	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; chb7Tg/chb7Tg	standard conditions	Fig. 6 from Huang et al., 2022
heart contraction normal process quality, ameliorated	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; chb7Tg/chb7Tg	standard conditions	Fig. 6 from Huang et al., 2022
pericardium normal object quality, ameliorated	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; chb7Tg/chb7Tg	standard conditions	Fig. 6 from Huang et al., 2022
bulbus arteriosus ab3-tnnt labeling spatial pattern, ameliorated	rbfox1l^chb5/chb5; rbfox2^chb6/chb6; chb7Tg/chb7Tg	standard conditions	Fig. 6 from Huang et al., 2022

Citations

Include unpublished citations

Huang, M., Akerberg, A.A., Zhang, X., Yoon, H., Joshi, S., Hallinan, C., Nguyen, C., Pu, W.T., Haigis, M.C., Burns, C.G., Burns, C.E. (2022) Intrinsic myocardial defects underlie an Rbfox-deficient zebrafish model of hypoplastic left heart syndrome. Nature communications. 13:5877

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