CRISPR

CRISPR1-leg1.1,leg1.2

ID

ZDB-CRISPR-230614-1

Previous Names

None

Targets

leg1.1 (1)
leg1.2 (1)

Sequence

5' - GGAGCAGGAGAATCCGCTC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zju22	leg1.2
zju23	leg1.1

Expression

Gene expression in Wild Types + CRISPR1-leg1.1,leg1.2

No data available

Phenotype

Phenotype resulting from CRISPR1-leg1.1,leg1.2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-leg1.1,leg1.2

Phenotype	Fish	Conditions	Figures
liver leg1.1 expression decreased amount, abnormal	leg1.2^zju22/zju22	standard conditions	Fig. 1 from Wang et al., 2021
liver leg1.2 expression decreased amount, abnormal	leg1.2^zju22/zju22	standard conditions	Fig. 1 from Wang et al., 2021
liver lipid metabolic process disrupted, abnormal	leg1.2^zju22/zju22 (AB)	standard conditions	Fig. 2, Fig. 3 from Wang et al., 2025
liver lipid droplet increased amount, abnormal	leg1.2^zju22/zju22 (AB)	standard conditions	Fig. 3 from Wang et al., 2025
liver fatty, abnormal	leg1.2^zju22/zju22 (AB)	standard conditions	Fig. 2, Fig. 3 from Wang et al., 2025
liver leg1.1 expression decreased amount, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22	standard conditions	Fig. 1 from Wang et al., 2021
whole organism decreased length, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22	standard conditions	Fig. 1 from Wang et al., 2021
whole organism Ab1-leg1 labeling absent, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22	standard conditions	Fig. 1 from Wang et al., 2021
whole organism decreased thickness, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22	standard conditions	Fig. 1 from Wang et al., 2021
liver leg1.2 expression decreased amount, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22	standard conditions	Fig. 1 from Wang et al., 2021
liver lipid metabolic process disrupted, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22 (AB)	standard conditions	Fig. 2, Fig. 3 from Wang et al., 2025
liver diglyceride increased amount, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22 (AB)	standard conditions	Fig. 4 from Wang et al., 2025
liver lipid droplet increased amount, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22 (AB)	standard conditions	Fig. 3 from Wang et al., 2025
yolk fatty, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22 (AB)	standard conditions	Fig. 2 from Wang et al., 2025
liver lipid droplet increased size, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22 (AB)	standard conditions	Fig. 3 from Wang et al., 2025
liver fatty, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22 (AB)	standard conditions	Fig. 2, Fig. 3 from Wang et al., 2025
blood plasma triglyceride increased amount, abnormal	leg1.1^zju23/zju23; leg1.2^zju22/zju22 (AB)	standard conditions	Fig. 4 from Wang et al., 2025

Citations