CRISPR

CRISPR1-strn3

ID

ZDB-CRISPR-230404-2

Name

CRISPR1-strn3

Previous Names

None

Target

strn3 (1)

Sequence

5' - GAGGAGGAATGGCCGCCCAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
pc45	strn3

Expression

Gene expression in Wild Types + CRISPR1-strn3

No data available

Phenotype

Phenotype resulting from CRISPR1-strn3

No data available

Phenotype of all Fish created by or utilizing CRISPR1-strn3

Phenotype	Fish	Conditions	Figures
skeletal muscle cell Z disc increased width, abnormal	strn3^pc45/pc45 (TU)	standard conditions	Fig. 7 from Berger et al., 2022
intertectal commissure axon extension decreased process quality, abnormal	strn3^pc45/pc45 (TU)	standard conditions	Fig. 7 from Berger et al., 2022
optic tectum axon extension decreased process quality, abnormal	strn3^pc45/pc45 (TU)	standard conditions	Fig. 7 from Berger et al., 2022
optic tectum has fewer parts of type intertectal commissure axon, abnormal	strn3^pc45/pc45 (TU)	standard conditions	Fig. 7 from Berger et al., 2022
optic tectum apoptotic process increased occurrence, abnormal	strn3^pc45/pc45 (TU)	standard conditions	Fig. 7 from Berger et al., 2022
whole organism strn3 expression decreased amount, abnormal	strn3^pc45/pc45 (TU)	standard conditions	Fig. 7 from Berger et al., 2022
retina apoptotic process increased occurrence, abnormal	strn3^pc45/pc45 (TU)	standard conditions	Fig. 7 from Berger et al., 2022
skeletal muscle cell sarcomere organization decreased process quality, abnormal	strn3^pc45/pc45 (TU)	standard conditions	Fig. 7 from Berger et al., 2022
skeletal muscle cell striated muscle thin filament disorganized, abnormal	strn3^pc45/pc45; pc21Tg; pc22Tg	standard conditions	Fig. 7 from Berger et al., 2022
myotome skeletal muscle myofibril decreased diameter, abnormal	strn3^pc45/pc45; pc21Tg; pc22Tg	standard conditions	Fig. 7 from Berger et al., 2022
myotome skeletal muscle myofibril disorganized, abnormal	strn3^pc45/pc45; pc21Tg; pc22Tg	standard conditions	Fig. 7 from Berger et al., 2022
skeletal muscle cell sarcomere organization decreased process quality, abnormal	mob4^pc43/pc43; strn3^pc45/pc45 (TU)	standard conditions	Fig. 7 from Berger et al., 2022
myotome skeletal muscle myofibril disorganized, abnormal	mob4^pc43/pc43; strn3^pc45/pc45 (TU)	standard conditions	Fig. 7 from Berger et al., 2022

Citations