CRISPR

CRISPR2-tpcn1

ID

ZDB-CRISPR-230317-5

Name

CRISPR2-tpcn1

Previous Names

None

Target

tpcn1 (1)

Sequence

5' - GGCGTATTCGAAGAATCGT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Genome Build: GRCz11Chromosome: 5

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
dhkz101	tpcn1

Expression

Gene expression in Wild Types + CRISPR2-tpcn1

No data available

Phenotype

Phenotype resulting from CRISPR2-tpcn1

No data available

Phenotype of all Fish created by or utilizing CRISPR2-tpcn1

Phenotype	Fish	Conditions	Figures
vertical myoseptum ab-f59 labeling spatial pattern, abnormal	tpcn1^{dhkz101/dhkz101}	standard conditions	Fig. 1 from Rice et al., 2022
trunk fast muscle cell ab-f59 labeling spatial pattern, abnormal	tpcn1^{dhkz101/dhkz101}	standard conditions	Fig. 1 from Rice et al., 2022
somite 8 vertical myoseptum ab3-dag1 labeling spatial pattern, abnormal	tpcn1^{dhkz101/dhkz101}	standard conditions	Fig. 6 from Rice et al., 2022
vertical myoseptum endolysosome irregular spatial pattern, abnormal	tpcn1^{dhkz101/dhkz101}	standard conditions	Fig. 5 from Rice et al., 2022
trunk slow muscle cell ab-f59 labeling spatial pattern, abnormal	tpcn1^{dhkz101/dhkz101}	standard conditions	Fig. 1, Fig. 2 from Rice et al., 2022
somite 9 vertical myoseptum ab3-dag1 labeling spatial pattern, abnormal	tpcn1^{dhkz101/dhkz101}	standard conditions	Fig. 6 from Rice et al., 2022
smooth muscle cell endolysosome irregular spatial pattern, abnormal	tpcn1^{dhkz101/dhkz101}	standard conditions	Fig. 5 from Rice et al., 2022
trunk slow muscle cell ab-f59 labeling spatial pattern, abnormal	AB + CRISPR1-tpcn1 + CRISPR2-tpcn1	standard conditions	Fig. 1, Fig. 2 from Rice et al., 2022
vertical myoseptum ab-f59 labeling spatial pattern, abnormal	AB + CRISPR1-tpcn1 + CRISPR2-tpcn1	standard conditions	Fig. 1, Fig. 2 from Rice et al., 2022
trunk fast muscle cell ab-f59 labeling spatial pattern, abnormal	AB + CRISPR1-tpcn1 + CRISPR2-tpcn1	standard conditions	Fig. 1 from Rice et al., 2022
vertical myoseptum ab-f59 labeling spatial pattern, abnormal	tpcn1^dhkz101/+	standard conditions	Fig. 1 from Rice et al., 2022
trunk fast muscle cell ab-f59 labeling spatial pattern, abnormal	tpcn1^dhkz101/+	standard conditions	Fig. 1 from Rice et al., 2022
trunk slow muscle cell ab-f59 labeling spatial pattern, abnormal	tpcn1^dhkz101/+	standard conditions	Fig. 1 from Rice et al., 2022

Citations

Include unpublished citations

Rice, K.L., Webb, S.E., Miller, A.L. (2022) Localized TPC1-mediated Ca2+ release from endolysosomes contributes to myoseptal junction development in zebrafish. Journal of Cell Science. 135(9):

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