CRISPR

CRISPR1-srrm4

ID

ZDB-CRISPR-230125-2

Name

CRISPR1-srrm4

Previous Names

None

Target

Sequence

5' - TGATTCTGCGGGCTTCCAGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
crg4	srrm4
crg6	srrm4

Expression

Gene expression in Wild Types + CRISPR1-srrm4

No data available

Phenotype

Phenotype resulting from CRISPR1-srrm4

No data available

Phenotype of all Fish created by or utilizing CRISPR1-srrm4

Phenotype	Fish	Conditions	Figures
visual system decreased functionality, exacerbated	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
retina mitochondrion fragmented, exacerbated	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
eye photoreceptor cell photoreceptor outer segment disorganized, abnormal	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
eye photoreceptor cell photoreceptor outer segment decreased length, abnormal	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
retina decreased functionality, exacerbated	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
whole organism dead, abnormal	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 4 from Ciampi et al., 2022
retina mitochondrion decreased size, exacerbated	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
retina mitochondrion decreased area, exacerbated	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022

Citations