CRISPR

CRISPR2-rasip1

ID

ZDB-CRISPR-221129-2

Name

CRISPR2-rasip1

Previous Names

Cris7 (1)

Target

rasip1 (1)

Sequence

5' - TGAAGCTCAGGGCTGGGGAT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ubs28	rasip1

Expression

Gene expression in Wild Types + CRISPR2-rasip1

No data available

Phenotype

Phenotype resulting from CRISPR2-rasip1

No data available

Phenotype of all Fish created by or utilizing CRISPR2-rasip1

Phenotype	Fish	Conditions	Figures
dorsal longitudinal anastomotic vessel lumen malformed, abnormal	rasip1^ubs28/ubs28; nkuasrfp1aTg; ubs3Tg	standard conditions	Fig. 5. from Lee et al., 2021
dorsal longitudinal anastomotic vessel has extra parts of type dorsal longitudinal anastomotic vessel intracellular vesicle, abnormal	rasip1^ubs28/ubs28; nkuasrfp1aTg; ubs3Tg	standard conditions	Fig. 5. from Lee et al., 2021
dorsal longitudinal anastomotic vessel blood vessel lumenization decreased process quality, abnormal	rasip1^ubs28/ubs28; nkuasrfp1aTg; ubs3Tg	standard conditions	Fig. 5. from Lee et al., 2021
blood circulation decreased process quality, abnormal	rasip1^ubs28/ubs28; nkuasrfp1aTg; ubs3Tg; uq11bhTg	standard conditions	Fig. 7. from Lee et al., 2021
dorsal longitudinal anastomotic vessel has extra parts of type dorsal longitudinal anastomotic vessel intracellular vesicle, abnormal	rasip1^ubs28/ubs28; nkuasrfp1aTg; ubs3Tg; uq11bhTg	standard conditions	Fig. 5. from Lee et al., 2021
dorsal longitudinal anastomotic vessel blood vessel lumenization decreased process quality, abnormal	rasip1^ubs28/ubs28; nkuasrfp1aTg; ubs3Tg; uq11bhTg	standard conditions	Fig. 5. from Lee et al., 2021
dorsal longitudinal anastomotic vessel lumen malformed, abnormal	rasip1^ubs28/ubs28; nkuasrfp1aTg; ubs3Tg; uq11bhTg	standard conditions	Fig. 5. from Lee et al., 2021
intersegmental vessel sprouting angiogenesis decreased process quality, abnormal	rasip1^ubs28/ubs28; s843Tg	standard conditions	Fig. 1. , Fig. 2. from Lee et al., 2021
dorsal longitudinal anastomotic vessel blood vessel lumenization decreased process quality, abnormal	rasip1^ubs28/ubs28; s843Tg	standard conditions	Fig. 5. from Lee et al., 2021
intersegmental vessel cell-cell junction assembly decreased process quality, abnormal	rasip1^ubs28/ubs28; s843Tg	standard conditions	Fig. 2. , Fig. 7. from Lee et al., 2021
dorsal longitudinal anastomotic vessel lumen closed, abnormal	rasip1^ubs28/ubs28; s843Tg	standard conditions	Fig. 5. from Lee et al., 2021
dorsal longitudinal anastomotic vessel lumen malformed, abnormal	rasip1^ubs28/ubs28; s843Tg	standard conditions	Fig. 5. from Lee et al., 2021
dorsal longitudinal anastomotic vessel lumen mislocalised, abnormal	rasip1^ubs28/ubs28; s843Tg	standard conditions	Fig. 5. from Lee et al., 2021
intersegmental vessel has fewer parts of type blood vessel endothelial cell, abnormal	rasip1^ubs28/ubs28; s843Tg	standard conditions	Fig. 1. from Lee et al., 2021
intersegmental vessel blood vessel lumenization delayed, abnormal	rasip1^ubs28/ubs28; s843Tg; sd2Tg	standard conditions	Fig. 4. from Lee et al., 2021
intersegmental vessel sprouting angiogenesis decreased process quality, abnormal	rasip1^ubs28/ubs28; uq11bhTg	standard conditions	Fig. 1. from Lee et al., 2021
intersegmental vessel cell-cell junction assembly decreased process quality, abnormal	rasip1^ubs28/ubs28; uq11bhTg	standard conditions	Fig. 1. from Lee et al., 2021
intersegmental vessel has fewer parts of type blood vessel endothelial cell, abnormal	rasip1^ubs28/ubs28; uq11bhTg	standard conditions	Fig. 1. from Lee et al., 2021
blood circulation decreased process quality, abnormal	radil2a^{sa20161/sa20161}; rasip1^ubs28/ubs28; nkuasrfp1aTg; ubs3Tg; uq11bhTg	standard conditions	Fig. 7. from Lee et al., 2021
blood circulation decreased process quality, exacerbated	radil2a^{sa20161/sa20161}; rasip1^ubs28/ubs28; nkuasrfp1aTg; ubs3Tg; uq11bhTg	standard conditions	Fig. 7. from Lee et al., 2021
intersegmental vessel cell-cell junction assembly decreased process quality, abnormal	radil2a^{sa20161/sa20161}; rasip1^ubs28/ubs28; s843Tg	standard conditions	Fig. 7. from Lee et al., 2021
intersegmental vessel cell-cell junction assembly decreased process quality, abnormal	rasip1^ubs28/ubs28; ncv27Tg	standard conditions	Fig. 8. from Lee et al., 2021
intersegmental vessel sprouting angiogenesis decreased process quality, abnormal	rasip1^ubs28/ubs28; ncv27Tg	standard conditions	Fig. 8. from Lee et al., 2021

Citations