CRISPR
CRISPR4-sox3
- ID
- ZDB-CRISPR-211110-1
- Name
- CRISPR4-sox3
- Previous Names
- None
- Target
- Sequence
-
5' - TCAAGGAGTCTTTCAAATGT - 3'
- Disclaimer
- Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
- Note
-
The PAM site was "GGG" at the 3' end.
- Genome Resources
- None
Target Location
Constructs
No data available
Genomic Features
Expression
Gene expression in Wild Types + CRISPR4-sox3
No data available
Phenotype
Phenotype resulting from CRISPR4-sox3
No data available
Phenotype of all Fish created by or utilizing CRISPR4-sox3
No data available
Citations
- Okada, K., Aoki, K., Tabei, T., Sugio, K., Imai, K., Bonkohara, Y., Kamachi, Y. (2022) Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9. Nucleic acids research. 50(5):2854-2871
- Ranawakage, D.C., Okada, K., Sugio, K., Kawaguchi, Y., Kuninobu-Bonkohara, Y., Takada, T., Kamachi, Y. (2021) Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor. Frontiers in cell and developmental biology. 8:598634
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