CRISPR

CRISPR1-sdhb

ID

ZDB-CRISPR-210716-2

Name

CRISPR1-sdhb

Previous Names

None

Target

sdhb (1)

Sequence

5' - GTTCACCGGCCCGCCGTCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Genome Build: GRCz11Chromosome: 22

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
rmc200	sdhb

Expression

Gene expression in Wild Types + CRISPR1-sdhb

No data available

Phenotype

Phenotype resulting from CRISPR1-sdhb

No data available

Phenotype of all Fish created by or utilizing CRISPR1-sdhb

Phenotype	Fish	Conditions	Figures
whole organism reactive oxygen species increased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Figure 1 from Dona et al., 2021
swim bladder absent, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 4 from Dona et al., 2020
locomotory behavior process quality, abnormal	sdhb^{rmc200/rmc200} (AB)	altered light dark cycle	Fig. 5 from Dona et al., 2020
startle response decreased process quality, abnormal	sdhb^{rmc200/rmc200} (AB)	control	Figure 5 from Dona et al., 2021
intestinal mucosa morphology, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. S3 from Dona et al., 2020
whole organism lactate increased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 6 from Dona et al., 2020
mitochondrial electron transport, succinate to ubiquinone process quality, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 6 from Dona et al., 2020
whole organism succinate increased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 6 from Dona et al., 2020
whole organism asparagine decreased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 6 from Dona et al., 2020
whole organism dead, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 3 from Dona et al., 2020
whole organism aspartate decreased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 6 from Dona et al., 2020
whole organism decreased life span, exacerbated	sdhb^{rmc200/rmc200} (AB)	chemical treatment by environment: L-ascorbic acid	Figure 3 from Dona et al., 2021
swim bladder decreased area, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 4 from Dona et al., 2020
swim bladder uninflated, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 4 from Dona et al., 2020
heart decreased functionality, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 5 from Dona et al., 2020
whole organism decreased life span, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Figure 3 from Dona et al., 2021 Fig. 3 from Dona et al., 2020
startle response decreased process quality, exacerbated	sdhb^{rmc200/rmc200} (AB)	chemical treatment by environment: L-ascorbic acid	Figure 5 from Dona et al., 2021
goblet cell increased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. S3 from Dona et al., 2020
muscle succinate increased amount, abnormal	sdhb^rmc200/+ (AB)	standard conditions	Fig. 4 from Miltenburg et al., 2024
startle response decreased process quality, abnormal	sdhb^rmc200/+ (AB)	chemical treatment by environment: L-ascorbic acid	Figure 5 from Dona et al., 2021
swim bladder decreased area, abnormal	sdhb^rmc200/+ (AB)	standard conditions	Fig. 4 from Dona et al., 2020

Citations

Include unpublished citations

No data available