CRISPR

CRISPR4-ush2a

ID

ZDB-CRISPR-210303-1

Name

CRISPR4-ush2a

Previous Names

None

Target

ush2a (1)

Sequence

5' - AATATCTGGCTGCACGGTCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3378	ush2a

Expression

Gene expression in Wild Types + CRISPR4-ush2a

No data available

Phenotype

Phenotype resulting from CRISPR4-ush2a

No data available

Phenotype of all Fish created by or utilizing CRISPR4-ush2a

Phenotype	Fish	Conditions	Figures
macula stereocilium bundle ush2a expression absent, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 7 from Toms et al., 2020
retinal rod cell rod photoreceptor outer segment ab5-rho labeling decreased amount, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 8 from Toms et al., 2020
retinal rod cell decreased amount, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 9 from Toms et al., 2020
retinal rod cell cell body ab5-rho labeling mislocalised, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 8 from Toms et al., 2020
visual perception decreased process quality, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 6 from Toms et al., 2020
photoreceptor outer segment layer lysosome increased amount, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 9 from Toms et al., 2020
photoreceptor cell lysosome increased amount, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 9 from Toms et al., 2020
retina photoreceptor cell ush2a expression absent, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 5 from Toms et al., 2020
retina ush2a expression decreased amount, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Fig. S4 from Toms et al., 2020
long single cone cell photoreceptor inner segment ab1-opn1sw2 labeling increased distribution, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 8 from Toms et al., 2020
anterior macula stereocilium bundle decreased width, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 7 from Toms et al., 2020
photoreceptor inner segment layer lysosome increased amount, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 9 from Toms et al., 2020
anterior macula stereocilium bundle decreased amount, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Figure 7 from Toms et al., 2020
retina ush2a expression decreased distribution, abnormal	ush2a^{zf3378/zf3378} (AB)	standard conditions	Fig. S4 from Toms et al., 2020

Citations