CRISPR

CRISPR1-paqr5b

ID
ZDB-CRISPR-210120-3
Name
CRISPR1-paqr5b
Previous Names
None
Target
Sequence
5' - GGTGTGGTAGGCGTTTCATG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "AGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
ecu15 paqr5b
szu4 paqr5b
Expression
Gene expression in Wild Types + CRISPR1-paqr5b
No data available
Phenotype
Phenotype resulting from CRISPR1-paqr5b
No data available
Phenotype of all Fish created by or utilizing CRISPR1-paqr5b
Phenotype Fish Conditions Figures
oocyte stage III oocyte maturation decreased occurrence, abnormal paqr5becu15/ecu15 (TU) chemical treatment by environment: (20R)-17,20-dihydroxypregn-4-en-3-one Fig. 1 from Wu et al., 2020
microvillous olfactory receptor neuron absence of anatomical entity, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 7 with image from Mustary et al., 2024
brain decreased length, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 3 with image from Mustary et al., 2024
cerebellum position, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 3 with image from Mustary et al., 2024
cerebellum mislocalised, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 3 with image from Mustary et al., 2024
trunk curved, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 1 with image from Mustary et al., 2024
brain morphology, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 1 with image from Mustary et al., 2024
frontal bone concave, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 2 with image from Mustary et al., 2024
brain decreased size, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 1 with image from Mustary et al., 2024
fertilized egg chorion decreased diameter, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 1 with image from Mustary et al., 2024
post-vent region curved, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 1 with image from Mustary et al., 2024
olfactory rosette cell decreased amount, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 7 with image from Mustary et al., 2024
olfactory rosette decreased size, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 4 with image from Mustary et al., 2024
whole organism viability, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 2 with image from Mustary et al., 2024
ciliated olfactory receptor neuron absence of anatomical entity, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 7 with image from Mustary et al., 2024
olfactory rosette anatomical structure quality, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 4 with image from Mustary et al., 2024
crypt olfactory receptor neuron absence of anatomical entity, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 7 with image from Mustary et al., 2024
autopalatine morphology, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 2 with image from Mustary et al., 2024
head morphology, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 1 with image,  Fig. 2 with image from Mustary et al., 2024
whole organism decreased fecundity, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 1 with image from Mustary et al., 2024
olfactory rosette paqr5b expression absent, abnormal paqr5bszu4/szu4; brsb2/b2; mpv17a9/a9 (AB) standard conditions Fig. 5 with image from Mustary et al., 2024
unfertilized egg chorion decreased size, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions Fig. 6 with image from Wu et al., 2020
ovulation disrupted, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
oocyte stage III mmp2 expression decreased amount, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
oocyte stage IV oocyte maturation delayed, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) chemical treatment by environment: (20R)-17,20-dihydroxypregn-4-en-3-one Fig. 2 with image from Wu et al., 2020
ovary has extra parts of type oocyte stage II, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions Fig. 7 with image from Wu et al., 2020
ovary has fewer parts of type oocyte stage V, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
oocyte stage IV mmp2 expression decreased amount, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
oocyte stage III lhcgr expression decreased amount, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
oocyte stage IV adamts1 expression decreased amount, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
whole organism decreased fertility, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
ovary decreased size, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
oocyte stage IV pgr expression decreased amount, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
oocyte stage IV oocyte maturation decreased occurrence, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) chemical treatment by environment: (20R)-17,20-dihydroxypregn-4-en-3-one Fig. 2 with image from Wu et al., 2020
oocyte stage IV lhcgr expression decreased amount, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
unfertilized egg yolk decreased size, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions Fig. 6 with image from Wu et al., 2020
oocyte stage IV oocyte maturation decreased rate of occurrence, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) chemical treatment by environment: (20R)-17,20-dihydroxypregn-4-en-3-one Fig. 2 with image from Wu et al., 2020
oocyte stage IV oocyte maturation increased occurrence, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) control Fig. 2 with image from Wu et al., 2020
ovary has extra parts of type oocyte stage I, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions Fig. 7 with image from Wu et al., 2020
whole organism brood size, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
oocyte stage III pgr expression decreased amount, abnormal paqr5aecu14/ecu14; paqr5becu15/ecu15; paqr6ecu16/ecu16; paqr7becu12/ecu12; paqr8ecu13/ecu13; paqr9ecu17/ecu17; paqr7aecu11/ecu11 (TU) standard conditions text only from Wu et al., 2020
yolk decreased size, abnormal paqr5aecu14; paqr5becu15; paqr6ecu16; paqr7becu12; paqr8ecu13; paqr9ecu17; paqr7aecu11 (TU) standard conditions Fig. 6 with image from Wu et al., 2020
yolk yolk granule disorganized, abnormal paqr5aecu14; paqr5becu15; paqr6ecu16; paqr7becu12; paqr8ecu13; paqr9ecu17; paqr7aecu11 (TU) standard conditions Fig. 6 with image from Wu et al., 2020
chorion decreased size, abnormal paqr5aecu14; paqr5becu15; paqr6ecu16; paqr7becu12; paqr8ecu13; paqr9ecu17; paqr7aecu11 (TU) standard conditions Fig. 6 with image from Wu et al., 2020
Citations
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