CRISPR

CRISPR1-mmut

ID

ZDB-CRISPR-200929-4

Name

CRISPR1-mmut

Previous Names

CRISPR1-mut

Target

mmut (1)

Sequence

5' - GGGCCAGCAGGGTCTGTCTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zh602	mmut

Expression

Gene expression in Wild Types + CRISPR1-mmut

No data available

Phenotype

Phenotype resulting from CRISPR1-mmut

No data available

Phenotype of all Fish created by or utilizing CRISPR1-mmut

Phenotype	Fish	Conditions	Figures
whole organism viability, abnormal	mmut^zh602/zh602 (TL, TU)	control	Fig. 9 from Luciani et al., 2020
kidney mitochondrion circular, abnormal	mmut^zh602/zh602 (TL, TU)	standard conditions	Fig. 3 from Luciani et al., 2020
kidney mitochondrion morphology, abnormal	mmut^zh602/zh602 (TL, TU)	standard conditions	Fig. 3 from Luciani et al., 2020
whole organism methylmalonic acid increased amount, abnormal	mmut^zh602/zh602 (TL, TU)	standard conditions	Fig. 3 , Fig. 9 , Fig. S4 from Luciani et al., 2020
swimming behavior process quality, ameliorated	mmut^zh602/zh602 (TL, TU)	low protein	Fig. 3 from Luciani et al., 2020
kidney mitochondrial crista disorganized, abnormal	mmut^zh602/zh602 (TL, TU)	standard conditions	Fig. 3 from Luciani et al., 2020
whole organism viability, abnormal	mmut^zh602/zh602 (TL, TU)	high protein	Fig. 3 from Luciani et al., 2020
swimming behavior decreased process quality, abnormal	mmut^zh602/zh602 (TL, TU)	high protein	Fig. 3 from Luciani et al., 2020
whole organism methylmalonic acid increased amount, abnormal	mmut^zh602/zh602 (TL, TU)	chemical treatment by environment: ubiquinones	Fig. 9 from Luciani et al., 2020
whole organism viability, ameliorated	mmut^zh602/zh602 (TL, TU)	chemical treatment by environment: ubiquinones	Fig. 9 from Luciani et al., 2020
swimming behavior decreased process quality, abnormal	mmut^zh602/zh602 (TL, TU)	control	Fig. 9 from Luciani et al., 2020
liver mitochondrion circular, abnormal	mmut^zh602/zh602 (TL, TU)	standard conditions	Fig. 3 from Luciani et al., 2020
liver mitochondrion morphology, abnormal	mmut^zh602/zh602 (TL, TU)	standard conditions	Fig. 3 from Luciani et al., 2020
aerobic respiration decreased process quality, abnormal	mmut^zh602/zh602 (TL, TU)	standard conditions	Fig. 3 from Luciani et al., 2020
liver mitochondrial crista disorganized, abnormal	mmut^zh602/zh602 (TL, TU)	standard conditions	Fig. 3 from Luciani et al., 2020
swimming behavior process quality, ameliorated	mmut^zh602/zh602 (TL, TU)	chemical treatment by environment: ubiquinones	Fig. 9 from Luciani et al., 2020
whole organism viability, ameliorated	mmut^zh602/zh602 (TL, TU)	low protein	Fig. 3 from Luciani et al., 2020
liver reactive oxygen species amount, ameliorated	mmut^zh602/+; zh604Tg	chemical treatment by environment: ubiquinones	Fig. 9 from Luciani et al., 2020
liver reactive oxygen species increased amount, abnormal	mmut^zh602/+; zh604Tg	standard conditions	Fig. 3 , Fig. 9 from Luciani et al., 2020
liver mitochondrion decreased functionality, abnormal	mmut^zh602/+; zh604Tg	standard conditions	Fig. 3 from Luciani et al., 2020
whole organism viability, ameliorated	mmut^zh602/zh602; zh603Tg	standard conditions	Fig. 3 from Luciani et al., 2020
whole organism methylmalonic acid amount, ameliorated	mmut^zh602/zh602; zh603Tg	standard conditions	Fig. S4 from Luciani et al., 2020

Citations