CRISPR

CRISPR1-pbx2,pbx3b

ID

ZDB-CRISPR-190327-1

Name

CRISPR1-pbx2,pbx3b

Previous Names

None

Targets

Sequence

5' - GGTGTAGCCGGGCCAGAGAA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

This CRISPR targets both pbx2(precisely), and pbx3b (with a single mismatch).

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
scm8	pbx3b

Expression

Gene expression in Wild Types + CRISPR1-pbx2,pbx3b

No data available

Phenotype

Phenotype resulting from CRISPR1-pbx2,pbx3b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-pbx2,pbx3b

Phenotype	Fish	Conditions	Figures
whole organism pbx3b expression decreased amount, abnormal	pbx3b^scm8/scm8 (AB)	standard conditions	Fig. 1 from Farr et al., 2018
embryonic heart tube morphogenesis decreased process quality, abnormal	pbx4^b557/b557; pbx3b^scm8/scm8 (AB)	standard conditions	Fig. 1 from Farr et al., 2018
whole organism pbx3b expression decreased amount, abnormal	pbx4^b557/b557; pbx3b^scm8/scm8 (AB)	standard conditions	Fig. 1 from Farr et al., 2018
myocardium malformed, abnormal	pbx4^b557/b557; pbx3b^scm8/scm8 (AB)	standard conditions	Fig. 1 from Farr et al., 2018
whole organism pbx4 expression decreased amount, abnormal	pbx4^b557/b557; pbx3b^scm8/scm8 (AB)	standard conditions	Fig. 1 from Farr et al., 2018
heart tube malformed, abnormal	pbx4^b557/b557; pbx3b^scm8/scm8 (AB)	standard conditions	Fig. 1 from Farr et al., 2018
cardiac muscle tissue morphogenesis decreased process quality, abnormal	pbx4^b557/b557; pbx3b^scm8/scm8 (AB)	standard conditions	Fig. 1 from Farr et al., 2018

Citations