CRISPR

CRISPR1-optn

ID

ZDB-CRISPR-190218-3

Name

CRISPR1-optn

Previous Names

None

Target

optn (1)

Sequence

5' - GCTGGAAAAAAGTGGAGCTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ibl51	optn

Expression

Gene expression in Wild Types + CRISPR1-optn

No data available

Phenotype

Phenotype resulting from CRISPR1-optn

No data available

Phenotype of all Fish created by or utilizing CRISPR1-optn

Phenotype	Fish	Conditions	Figures
defense response to bacterium decreased efficacy, abnormal	optn^ibl51/ibl51	bacterial treatment by injection: Mycobacterium marinum	Figure 1 , Figure 4 from Xie et al., 2024
defense response to bacterium decreased efficacy, exacerbated	optn^ibl51/ibl51; sqstm1^ibl52/ibl52	bacterial treatment by injection: Mycobacterium marinum	Figure 1 from Xie et al., 2024
defense response to bacterium decreased efficacy, abnormal	optn^ibl51/ibl51; sqstm1^ibl52/ibl52	bacterial treatment by injection: Mycobacterium marinum	Figure 4 from Xie et al., 2024
whole organism ab1-map1lc3 labeling amount, ameliorated	optn^ibl51/ibl51; zf155Tg	bacterial treatment by injection: Mycobacterium marinum	Fig 5 from Zhang et al., 2019
whole organism optn expression decreased amount, abnormal	optn^ibl51/ibl51; zf155Tg	control	Fig. 2 from Zhang et al., 2019
caudal fin EGFP expression amount, ameliorated	optn^ibl51/ibl51; zf155Tg	chemical treatment by environment: bafilomycin A1	Fig 3 from Zhang et al., 2019
whole organism ab2-optn labeling decreased amount, abnormal	optn^ibl51/ibl51; zf155Tg	control	Fig. 2 from Zhang et al., 2019
whole organism ab1-map1lc3 labeling amount, ameliorated	optn^ibl51/ibl51; zf155Tg	chemical treatment by environment: bafilomycin A1	Fig 3 from Zhang et al., 2019
defense response to bacterium decreased efficacy, abnormal	optn^ibl51/ibl51; zf155Tg	bacterial treatment by injection: Mycobacterium marinum	Fig. 4 from Zhang et al., 2019
defense response to bacterium decreased efficacy, abnormal	optn^ibl51/ibl51; zf155Tg + MO1-sqstm1	bacterial treatment by injection: Mycobacterium marinum	Fig. 4 from Zhang et al., 2019

Citations