CRISPR

CRISPR1-pxnb

ID

ZDB-CRISPR-170609-11

Name

CRISPR1-pxnb

Previous Names

None

Target

pxnb (1)

Sequence

5' - GAGCTCCTCAAGTTCTCGTGTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sny7100	pxnb
sny7300	pxnb

Expression

Gene expression in Wild Types + CRISPR1-pxnb

No data available

Phenotype

Phenotype resulting from CRISPR1-pxnb

No data available

Phenotype of all Fish created by or utilizing CRISPR1-pxnb

Phenotype	Fish	Conditions	Figures
myotome development decreased process quality, abnormal	pxnb^{sny7100/sny7100}	standard conditions	Fig. 4 from Jacob et al., 2017
myotome malformed, abnormal	pxnb^{sny7100/sny7100}	standard conditions	Fig. 4 from Jacob et al., 2017
whole organism pxna expression absent, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 2 from Jacob et al., 2017
myotome muscle tendon junction ab1-lama1 labeling decreased amount, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 5 from Jacob et al., 2017
myotome muscle cell increased length, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 4 from Jacob et al., 2017
myotome muscle tendon junction ab2-fn labeling increased distribution, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 5 from Jacob et al., 2017
notochord outer sheath cell cell-cell junction ab1-tjp1 labeling mislocalised, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 3 from Jacob et al., 2017
cardiac ventricle decreased volume, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 3 from Jacob et al., 2017
post-vent region notochord kinked, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 2 from Jacob et al., 2017
brain edematous, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 3 from Jacob et al., 2017
cardiac ventricle decreased size, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 3 from Jacob et al., 2017
notochord malformed, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 3 from Jacob et al., 2017
whole organism decreased length, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 2 from Jacob et al., 2017
myotome malformed, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 4 , Fig. 5 from Jacob et al., 2017
heart looping decreased process quality, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 3 from Jacob et al., 2017
whole organism pxnb expression absent, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 2 from Jacob et al., 2017
heart edematous, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 2 , Fig. 3 from Jacob et al., 2017
myotome muscle tendon junction ab2-fn labeling increased amount, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 5 from Jacob et al., 2017
heart edematous, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 2 , Fig. 3 from Jacob et al., 2017
heart looping decreased process quality, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 3 from Jacob et al., 2017
notochord outer sheath cell cytoplasm ab1-tjp1 labeling mislocalised, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 3 from Jacob et al., 2017
brain edematous, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 3 from Jacob et al., 2017
myotome muscle cell detached from myotome muscle tendon junction, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 4 , Fig. 5 from Jacob et al., 2017
myotome development decreased process quality, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 4 from Jacob et al., 2017
myotome development decreased process quality, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 4 , Fig. 5 from Jacob et al., 2017
vertical myoseptum ab1-lama1 labeling decreased amount, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 5 from Jacob et al., 2017
notochord outer sheath cell mislocalised, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 3 from Jacob et al., 2017
myotome malformed, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 4 from Jacob et al., 2017
heart edematous, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 2 from Jacob et al., 2017
atrium decreased volume, abnormal	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}	standard conditions	Fig. 3 from Jacob et al., 2017

Citations