Genomic Feature
m216
- ID
- ZDB-ALT-980203-542
- Name
- m216
- Synonyms
- None
- Affected Genomic Region
- Construct
- None
- Type
- Allele with one point mutation (1)
- Protocol
- adult males treated with ENU
- Lab of Origin
- Driever Lab
- Current Source
- Zebrafish International Resource Center (ZIRC) ( order this )
- Other Pages
Notes
No data available
Variants
- Variant Type
- Point Mutation
- Variant Location
- Chr 21: 13687101 (GRCz11) (1) Details
- Nucleotide change
- A/G
- Variant Notes
-
The m216 and e713 alleles show the same base change.
Effect on DNA/cDNA, transcript, protein (from publications)
- DNA/cDNA Change
- T>C (1)
- Transcript Consequence
- Missense (1)
- Protein Consequence
- Amino Acid Substitution: Leu>Pro (1)
- Flanking Sequence
-
ATAATAAGCATGGCTGATTTGATCCATAGCTTGGCTTGGAGCTTTTCTATAAACTTTAGTGAGCACCTGGAGCAAGTTAGCTGGTGAGATGACCCACCAAACCAGGGTGCAAGCCGTTTTTGAAGACATCTGGTCGGTGGAGGGATGGCATGTATAAGGCAGGGGCTCCTCCAGGATGGGCTGGTCCAGGATAGCCTTGACCTGGGAGCACAGTGCCACCCATGTGGGGTGGAGGTGGTGGACTCTGCTCGTAATACTGTGCTTCAACACACTCGTCATCAAAGGGCAAAGCTAGACGCTTTCCCTTCTGTCTACGGTTGCAGAACCATACACGCACTACCTAGAGAAAAGCACATTGTTAAAGTCAAACAGCTTCTAGAAGGTGAAAAATGCTTCACAGACTTTGAGGCGTGCTTACATCTCTCTCCAGGCCTAGATCATCGGATATGTGCGTTATCTCCAGAGTGTTGGGTTTGGGGCACTTCACGAAGTACGACTCT
A/G GAGCAGAACGGACTGTGCCTTCCAAGCTGGTCCTTCGTTTTCTTTTTCGCGTGTCGACAAACACCCGTTCAATTTTGTACATCTGAAAGATAAATTGAAATAACATTAAGCTCAAAATTAAGCTTGTTTGACATAAATCTTTTGATAAAACCAAAGGTTAAGAGTAACAAATAAATTTTTTGCAAGTACATGGAATCATTTATGTTGTTGGCCAATCAATGCAATAAGTGGTCATTTGTTTTAGATTATAGCCTGTACAACAAATATACTTGATATGCTAGTGTGAAGATTTGTTTTCTCGTGTGAGAAAATGTAGACCACTCAACATTGTCATACACTTAAGAGTATTATACCAAAACATTTTTTCACAGTTTGTGTTTGATGTGCATAAGGGTTTCATTATGAGACATTCAACCTCCACACTAAAAATTGAATGGTGACTTTAAATGTATTTTACAACTGCTAAATGTCAAGAGTTCAGATGCAAAAACCTCTAAATG - Additional Sequence
- None
Fish
| Fish | Genomic Feature Zygosity | Parental Zygosity | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|---|---|
| pou5f3m216/m216 (AB) | Homozygous | Unknown | 5 figures  from 4 publications | 2 figures from Schier et al., 1996 | |
| pou5f3m216/+ (AB) | Heterozygous | Unknown |
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Supplemental Information
- Genotyping protocol
- None
- Burgess, S., Reim, G., Chen, W., Hopkins, N., and Brand, M. (2002) The zebrafish spiel-ohne-grenzen (spg) gene encodes the POU domain protein Pou2 related to mammalian Oct4 and is essential for formation of the midbrain and hindbrain, and for pre-gastrula morphogenesis. Development (Cambridge, England). 129(4):905-916
- Hauptmann, G., Belting, H.-G., Wolke, U., Lunde, K., Söll, I., Abdelilah-Seyfried, S., Prince, V., and Driever, W. (2002) spiel ohne grenzen/pou2 is required for zebrafish hindbrain segmentation. Development (Cambridge, England). 129(7):1645-1655
- Reim, G. and Brand, M. (2002) spiel-ohne-grenzen/pou2 mediates regional competence to respond to Fgf8 during zebrafish early neural development. Development (Cambridge, England). 129(4):917-933
- Belting, H.G., Hauptmann, G., Meyer, D., Abdelilah-Seyfried, S., Chitnis, A., Eschbach, C., Söll, I., Thisse, C., Thisse, B., Artinger, K., Lunde, K., and Driever, W. (2001) spiel ohne grenzen/pou2 is required during establishment of the zebrafish midbrain-hindbrain boundary organizer. Development (Cambridge, England). 128(21):4165-4176
- Reifers, F., Adams, J., Mason, I.J., Schulte-Merker, S., and Brand, M. (2000) Overlapping and distinct functions provided by fgf17, a new zebrafish member of the Fgf8/17/18 subgroup of Fgfs. Mechanisms of Development. 99(1-2):39-49
- Brand, M., Heisenberg, C.P., Jiang, Y.J., Beuchle, D., Lun, K., Furutani-Seiki, M., Granato, M., Haffter, P., Hammerschmidt, M., Kane, D.A., Kelsh, R.N., Mullins, M.C., Odenthal, J., van Eeden, F.J., and Nüsslein-Volhard, C. (1996) Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. Development (Cambridge, England). 123:179-190
- Brand, M., Heisenberg, C.P., Warga, R.M., Pelegri, F., Karlstrom, R.O., Beuchle, D., Picker, A., Jiang, Y.J., Furutani-Seiki, M., van Eeden, F.J., Granato, M., Haffter, P., Hammerschmidt, M., Kane, D.A., Kelsh, R.N., Mullins, M.C., Odenthal, J., and Nüsslein-Volhard, C. (1996) Mutations affecting development of the midline and general body shape during zebrafish embryogenesis. Development (Cambridge, England). 123:129-142
- Schier, A.F., Neuhauss, S.C., Harvey, M., Malicki, J., Solnica-Krezel, L., Stainier, D.Y., Zwartkruis, F., Abdelilah, S., Stemple, D.L., Rangini, Z., Yang, H., and Driever, W. (1996) Mutations affecting the development of the embryonic zebrafish brain. Development (Cambridge, England). 123:165-178
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