Genomic Feature
um27
- ID
- ZDB-ALT-111201-1
- Name
- um27
- Synonyms
- None
- Affected Genomic Region
- Construct
- None
- Type
- Allele with one deletion (1)
- Protocol
- embryos treated with zinc finger nuclease
- Lab of Origin
- Lawson Lab
- Current Source
- Other Pages
Notes
No data available
Variants
- Variant Type
- Small Deletion
- Variant Location
- Chr 11: 3853957 - 3853966 (GRCz11) (1) Details
- Nucleotide change
- GCCACAGCGG/-
- Variant Notes
Effect on DNA/cDNA, transcript, protein (from publications)
- DNA/cDNA Change
- 10 bp deleted (1)
- Transcript Consequence
- Frameshift, Premature Stop (1)
- Protein Consequence
- None
- Flanking Sequence
-
AATGGAACCAGAATGGTATAAGCCAACCACACAAAGCAAAAACCAATGGCATGTGGCTTTCTAAACACCCCTCAGCCAGATCCAAACAGATGCGTCCAGGCAATATTGCCGCAATTTTTTTTTCTGTCATTGTGGAGTGGTGATAATTGTACTGGCATGGGCATTGGTATCAGTGACAGTATCAGAAGTAGAAGCAATAATGATAGCGGGAACAATAGCAATAGTAGCAGTATTGTAACAGGCAAAGTAGGGGATCACCAGGGGGTGAGGGCGACAAACAATTCCAGGAAGATTATAGGGAAAATATAACTTTCCTGGAATTGTGTTCTGCTTCGTGCTCCTATTTAGCCCTCTTTCTCACGTGTGTGTGTTTGGGGGAACTGAAAGTACTCATCTACTCACCAGTCTGCGCTTTGGCTTGATAAGGGGTCTGTTCTGGCCGTTCATCTTGTGGTACAGGCCGCACGCGTTGCAGAGGTAGTGGCCCGTTCCATCCCGCC
GCCACAGCGG/- GGTGGAGGTGGCTCCACAGTTCACACATTCACGCCCCTCTGTGCAGAATTCAAGTGGAGGAAAAGCAAAGGGAAATTTATATAATTAAGAGGTTCACACTCTGATACAATACAGGAACACAGGGTCACGGAAAAGCCACGTTCTGGCCATAGAAAAACAACTCCCAAGTTAATTAGCTAATATCATGACATTTTCACATTGGATTTTTGCATCAGATCTGGATGTTACACTTTTCATGTCTGCAGAGTCCTAAAAAATAACATTTTAGGCCTTAAAAGGTCTTAAATCAACTGAAATATTGTGTTTTAGGTCCTAAATCGTTATTTTAACAGGTCTTAATTTCGCTTTGTTCATGTATAGCTACCCAATCTGGGCAACACCTGTTCAATCACCAACAATCCACGTCAATAACAAGATTTTTTTTTTTTACTTAATTTTGACTATATTTACTATTTTGATTTAATTACTTTATTTATAATAAAATTTGTTTGAAAGTTCTC - Additional Sequence
- None
Fish
| Fish | Genomic Feature Zygosity | Parental Zygosity | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|---|---|
| gata2aum27/um27 | Homozygous | Unknown | 3 figures  from Peña et al., 2021 | 3 figures from Peña et al., 2021 | |
| gata2aum27/um27 | Homozygous | ♀+/- ♂+/- | 10 figures from 5 publications | 7 figures from 2 publications | |
| gata2aum27/+ | Heterozygous | Unknown | 3 figures from Peña et al., 2021 | 3 figures from Peña et al., 2021 | |
| gata2aum27/+; la2Tg/la2Tg | Complex | FIGURE 2 from Peña et al., 2021 | |||
| gata2aum27/um27; ig11Tg; s957Tg | Complex | 2 figures from Gálvez-Santisteban et al., 2019 | |||
| gata2aum27/um27; la2Tg/la2Tg | Complex | FIGURE 2 from Peña et al., 2021 | |||
| gata2aum27/um27; la116Tg | Complex | Fig. 6 from Zhu et al., 2011 | Fig. 6 from Zhu et al., 2011 | ||
| gata2aum27/um27; mw43Tg; s957Tg | Complex | 2 figures from Gálvez-Santisteban et al., 2019 | |||
| gata2aum27/um27; s957Tg | Complex | Figure 4 from Gálvez-Santisteban et al., 2019 | 2 figures from Gálvez-Santisteban et al., 2019 | ||
| gata2aum27/um27; sd2Tg; zf72Tg | Complex | Fig. S1 from Butko et al., 2015 | Fig. S1 from Butko et al., 2015 |
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Supplemental Information
- Genotyping protocol
- None
- Shin, M., Yin, H.M., Shih, Y.H., Nozaki, T., Portman, D., Toles, B., Kolb, A., Luk, K., Isogai, S., Ishida, K., Hanasaka, T., Parsons, M.J., Wolfe, S.A., Burns, C.E., Burns, C.G., Lawson, N.D. (2023) Generation and application of endogenously floxed alleles for cell-specific knockout in zebrafish. Developmental Cell. 58(22):2614-2626.e7
- Peña, O.A., Lubin, A., Rowell, J., Hoade, Y., Khokhar, N., Lemmik, H., Mahony, C., Dace, P., Umamahesan, C., Payne, E.M. (2021) Differential Requirement of Gata2a and Gata2b for Primitive and Definitive Myeloid Development in Zebrafish. Frontiers in cell and developmental biology. 9:708113
- Gálvez-Santisteban, M., Chen, D., Zhang, R., Serrano, R., Nguyen, C., Zhao, L., Nerb, L., Masutani, E.M., Vermot, J., Burns, C.G., Burns, C.E., Del Álamo, J.C., Chi, N.C. (2019) Hemodynamic-mediated endocardial signaling controls in vivo myocardial reprogramming. eLIFE. 8:
- Shin, M., Nozaki, T., Idrizi, F., Isogai, S., Ogasawara, K., Ishida, K., Yuge, S., Roscoe, B., Wolfe, S.A., Fukuhara, S., Mochizuki, N., Deguchi, T., Lawson, N.D. (2019) Valves Are a Conserved Feature of the Zebrafish Lymphatic System. Developmental Cell. 51(3):374-386.e5
- Andrzejczuk, L.A., Banerjee, S., England, S.J., Voufo, C., Kamara, K., Lewis, K.E. (2018) Tal1, Gata2a, and Gata3 Have Distinct Functions in the Development of V2b and Cerebrospinal Fluid-Contacting KA Spinal Neurons. Frontiers in neuroscience. 12:170
- Butko, E., Distel, M., Pouget, C., Weijts, B., Kobayashi, I., Ng, K., Mosimann, C., Poulain, F.E., McPherson, A., Ni, C.W., Stachura, D.L., Del Cid, N., Espín-Palazón, R., Lawson, N.D., Dorsky, R., Clements, W.K., Traver, D. (2015) Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo. Development (Cambridge, England). 142:1050-61
- Kok, F.O., Shin, M., Ni, C., Gupta, A., Grosse, A.S., van Impel, A., Kirchmaier, B.C., Peterson-Maduro, J., Kourkoulis, G., Male, I., DeSantis, D.F., Sheppard-Tindell, S., Ebarasi, L., Betsholtz, C., Schulte-Merker, S., Wolfe, S.A., Lawson, N.D. (2015) Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish. Developmental Cell. 32(1):97-108
- Zhu, C., Smith, T., McNulty, J., Rayla, A.L., Lakshmanan, A., Siekmann, A.F., Buffardi, M., Meng, X., Shin, J., Padmanabhan, A., Cifuentes, D., Giraldez, A.J., Look, A.T., Epstein, J.A., Lawson, N.D., and Wolfe, S.A. (2011) Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development (Cambridge, England). 138(20):4555-4564
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