IDENTIFICATION OF RAPD PRIMERS THAT REVEAL EXTENSIVE POLYMORPHISMS BETWEEN LABORATORY STRAINS OF ZEBRAFISH
By S.L. Johnson, C.N. Midson, E.W. Ballinger, and J.H. Postlethwait; Institute of Neuroscience, University of Oregon, Eugene, OR 97403-1254
The zebrafish has recently emerged as a useful system for understanding vertebrate developmental genetics, despite the lack of a linkage map. To identify DNA-based genetic polymorphisms for constructing a genetic map, we have screened a collection of RAPD primers for their utility in identifying genetic polymorphisms between two laboratory strains of zebrafish. Here, we report 116 primers that identify 721 strain-specific genetic markers and show how they can be use din haploid genetics of zebrafish.
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