control
- full range of oocyte maturation stages
day 3 of exposure to 10 ng/L EE2
- extensive atresia of vitellogenic oocytes
- only previtellogenic oocytes remain vital
day 6 of exposure to 10 ng/L EE2
- atretic oocytes are visible as atretic follicles
- only previtellogenic oocytes remain vital
- intravascular plasma formation and extravascular fluid building, due to vitellogenin overproduction and subsequent osmotic changes
day 12 of exposure to 10 ng/L EE2
- atretic follicles are cleared away
- only previtellogenic oocytes remain vital
- intravascular plasma formation and extravascular fluid building, due to vitellogenin overproduction and subsequent osmotic changes
day 24 of exposure to 10 ng/L EE2
- extensive accumulation of peritoneal proteinaceous fluid and intensely staining plasma, due to vitellogenin overproduction and subsequent osmotic changes
- atretic follicles are largely cleared away
- only previtellogenic oocytes remain vital
day 6 postexposure (of 24 days)
- no histologic recovery
- extensive peritoneal fluid formation, due to vitellogenin overproduction and subsequent osmotic changes
- atretic follicles are cleared away
- only previtellogenic oocytes remain vital
adult female zebrafish, H&E (all images) day 24 postexposure (of 24 days)
- full histologic recovery
- full range of oocyte maturation stages (previtellogenic and vitellogenic)
- no accumulation of atretic follicles
- no aberrant plasma or extravascular fluid building
Reference
- van den Belt-K, Wester-PW, van der Ven-LTM, Verheyen-R, and Witters-H. Time-dependent effects of ethynylestradiol on the reproductive physiology in zebrafish (Danio rerio). Environ. Toxicol. Chem. 21:767-77; 2002.