ZFIN Lab: Laboratory of Dr. Andrew L. Miller

ZFIN ID: ZDB-LAB-970424-45

Laboratory of Dr. Andrew L. Miller

PI/Director:	Miller, Andrew L.
Contact Person:
Email:	almiller@ust.hk
URL:	http://home.ust.hk/~aequorin/calcium.htm
Address:	HKUST Calcium-Aequorin Imaging Laboratory Department of Biology The Hong Kong University of Science and Technology Clear Water Bay Kowloon HONG KONG
Country:	Hong Kong SAR China
Phone:	852-2358-7324
Fax:	852-2358-7323
Line Designation:	hkz

GENOMIC FEATURES ORIGINATING FROM THIS LAB

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STATEMENT OF RESEARCH INTERESTS

We are studying the role played by calcium ions in a wide range of fundamental biological processes. Calcium signaling is studied through the use of aequorins, a group of bioluminescent proteins. They can either be microinjected into cells or transgenically expressed without disturbing function or development. The pattern of luminescence that is emitted by an aequorin-loaded cell, tissue or organ, reveals changing patterns and levels of free calcium. We are currently studying cytokinesis in frog and fish eggs; cell cycle control in sea urchin and parchment worm eggs; injury and degeneration in squid, fish and mouse neurons, and differentiation in slime molds.

LAB MEMBERS

Webb, Sarah E. Post-Doc	Cheng, Chong Nam Graduate Student	Cheung, Yuk Kam Chris Graduate Student
Ma, Leung-Hang Omicron Graduate Student	Tse, Pui Ying Fish Facility Staff	Yim, Yun Fong Fish Facility Staff

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Rice, K.L., Chan, C.M., Kelu, J.J., Miller, A.L., Webb, S.E. (2023) A Role for Two-Pore Channel Type 2 (TPC2)-Mediated Regulation of Membrane Contact Sites During Zebrafish Notochord Biogenesis?. Contact (Thousand Oaks (Ventura County, Calif.)). 6:2515256423121140925152564231211409

Cheng, J.C., Miller, A.L., Webb, S.E. (2023) Actin-mediated endocytosis in the E-YSL helps drive epiboly in zebrafish. Zygote (Cambridge, England). 31(6):517-526

Choi, S.S.A., Chan, H.H., Chan, C.M., Wang, X., Webb, S.E., Leung, K.W., Tsim, K.W.K., Miller, A.L. (2022) Neuromasts and Olfactory Organs of Zebrafish Larvae Represent Possible Sites of SARS-CoV-2 Pseudovirus Host Cell Entry. Journal of virology. 96(24):e0141822

Lin, S., Wang, X., Tang, R.W., Lee, H.C., Chan, H.H., Choi, S.S.A., Dong, T.T., Leung, K.W., Webb, S.E., Miller, A.L., Tsim, K.W. (2022) The Extracts of Polygonum cuspidatum Root and Rhizome Block the Entry of SARS-CoV-2 Wild-Type and Omicron Pseudotyped Viruses via Inhibition of the S-Protein and 3CL Protease. Molecules. 27(12)

Wang, X., Lin, S., Tang, R.W., Lee, H.C., Chan, H.H., Choi, S.S.A., Leung, K.W., Webb, S.E., Miller, A.L., Tsim, K.W. (2022) Polygoni multiflori radix extracts inhibit SARS-CoV-2 pseudovirus entry in HEK293T cells and zebrafish larvae. Phytomedicine : international journal of phytotherapy and phytopharmacology. 102:154154

Rice, K.L., Webb, S.E., Miller, A.L. (2022) Localized TPC1-mediated Ca2+ release from endolysosomes contributes to myoseptal junction development in zebrafish. Journal of Cell Science. 135(9):

Fong, C.K., Hearn, T., Webb, S.E., Frøland Steindal, I.A., Miller, A.L., Whitmore, D. (2021) Daily rhythms in heartbeat rate are intrinsic to the zebrafish heart. Current biology : CB. 31:R239-R240

Yang, L., Webb, S.E., Jin, N., Lee, H.M., Chan, T.F., Xu, G., Chan, J.C.N., Miller, A.L., Ma, R.C.W. (2021) Investigating the role of dachshund b in the development of the pancreatic islet in zebrafish. Journal of diabetes investigation. 12(5):710-727

Jamieson, L., Waters, A., Ho, K.E., Chan, H.Y.S., Hung, J.T., Webb, S.E., Chan, C.M., Shipley, A.M., Williamson, J.G., Beer, J., Angus, C., Miller, A.L. (2020) Short-term homeostatic regulation of blood/interstitial fluid Ca²⁺ concentration by the scales of anadromous sea trout Salmo trutta L. during smoltification and migration. Journal of Fish Biology. 98(1):17-32

Guo, C., Webb, S.E., Chan, C.M., Miller, A.L. (2020) TPC2-mediated Ca²⁺ signaling is required for axon extension in caudal primary motor neurons in zebrafish embryos. Journal of Cell Science. 133(13):

Johnson, R.E., Linderman, S., Panier, T., Wee, C.L., Song, E., Herrera, K.J., Miller, A., Engert, F. (2019) Probabilistic Models of Larval Zebrafish Behavior Reveal Structure on Many Scales. Current biology : CB. 30(1):70-82.e4

Kelu, J.J., Webb, S.E., Galione, A., Miller, A.L. (2018) Characterization of ADP-ribosyl cyclase 1-like (ARC1-like) activity and NAADP signaling during slow muscle cell development in zebrafish embryos. Developmental Biology. 445(2):211-225

Tse, M.K., Hung, T.S., Chan, C.M., Wong, T., Dorothea, M., Leclerc, C., Moreau, M., Miller, A.L., Webb, S.E. (2018) Identification of Ca²⁺ signaling components in neural stem/progenitor cells during differentiation into neurons and glia in intact and dissociated zebrafish neurospheres.. Science China. Life sciences. 61(11):1352-1368

Xu, S., Webb, S.E., Lau, T.C.K., Cheng, S.H. (2018) Matrix metalloproteinases (MMPs) mediate leukocyte recruitment during the inflammatory phase of zebrafish heart regeneration. Scientific Reports. 8:7199

Kelu, J.J., Webb, S.E., Galione, A., Miller, A.L. (2018) TPC2-mediated Ca²⁺ signaling is required for the establishment of synchronized activity in developing zebrafish primary motor neurons.. Developmental Biology. 438(1):57-68

Kelu, J.J., Webb, S.E., Parrington, J., Galione, A., Miller, A.L. (2017) Ca²⁺ release via two-pore channel type 2 (TPC2) is required for slow muscle cell myofibrillogenesis and myotomal patterning in intact zebrafish embryos.. Developmental Biology. 425(2):109-129

Li, W.M., Chan, C.M., Miller, A.L., Lee, C.H. (2017) Dual Functional Roles of Molecular Beacon: a MicroRNA Detector and Inhibitor. The Journal of biological chemistry. 292(9):3568-3580

Webb, S.E., Miller, A.L. (2017) Ca²⁺ Signalling and Membrane Dynamics During Cytokinesis in Animal Cells.. Advances in experimental medicine and biology. 981:389-412

Chan, C.M., Aw, J.T., Webb, S.E., Miller, A.L. (2016) SOCE proteins, STIM1 and Orai1, are localized to the cleavage furrow during cytokinesis of the first and second cell division cycles in zebrafish embryos. Zygote (Cambridge, England). 24(6):880-889

Webb, S. (2016) LARGER, SHARPER & DEEPER. Biotechniques. 61:285-291

Barnes, M., Rensburg, G.V., Li, W.M., Mehmood, K., Mackedenski, S., Chan, C.M., King, D.T., Miller, A.L., Lee, C.H. (2015) Molecular Insights into the Coding Region Determinant-Binding Protein-RNA Interaction through Site-Directed Mutagenesis in the Heterogeneous Nuclear Ribonucleoprotein-K-homology Domains. The Journal of biological chemistry. 290(1):625-39

Kelu, J.J., Chan, H.L., Webb, S.E., Cheng, A.H., Ruas, M., Parrington, J., Galione, A., Miller, A.L. (2015) Two-Pore Channel 2 activity is required for slow muscle cell-generated Ca²⁺ signaling during myogenesis in intact zebrafish. The International journal of developmental biology. 59:313-325

Ma, L.H., Grove, C.L., Baker, R. (2014) Development of oculomotor circuitry independent of hox3 genes. Nature communications. 5:4221

Webb, S.E., Goulet, C., Chan, C.M., Yuen, M.Y., and Miller, A.L. (2014) Biphasic assembly of the contractile apparatus during the first two cell division cycles in zebrafish embryos. Zygote (Cambridge, England). 22:218-228

Yuen, M.Y., Webb, S.E., Chan, C.M., Thisse, B., Thisse, C., and Miller, A.L. (2013) Characterization of Ca(2+) signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development. Biochimica et biophysica acta. Molecular cell research. 1833(7):1641-56

Chan, C.M., Miller, A.L., and Webb, S.E. (2013) Reconstitution of holo-aequorin with apoaequorin mRNA and coelenterazine in zebrafish embryos. Cold Spring Harbor protocols. 2013(5):pdb.prot072975

Webb, S.E., and Miller, A.L. (2013) Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos. Cold Spring Harbor protocols. 2013(5):pdb.prot072967

Webb, S.E., Chan, C.M., and Miller, A.L. (2013) Introduction of Aequorin into Zebrafish Embryos for Recording Ca(2+) Signaling during the First 48 h of Development. Cold Spring Harbor protocols. 2013(5):383-6

Webb, S.E., and Miller, A.L. (2013) Ca(2+) signaling during activation and fertilization in the eggs of teleost fish. Cell Calcium. 53(1):24-31

Bianco, I.H., Ma, L.H., Schoppik, D., Robson, D.N., Orger, M.B., Beck, J.C., Li, J.M., Schier, A.F., Engert, F., and Baker, R. (2012) The Tangential Nucleus Controls a Gravito-inertial Vestibulo-ocular Reflex. Current biology : CB. 22(14):1285-1295

Webb, S.E., Cheung, C.C., Chan, C.M., Love, D.R., and Miller, A.L. (2012) The application of complementary luminescent and fluorescent imaging techniques to visualize nuclear and cytoplasmic Ca²⁺-signalling during the in vivo differentiation of slow muscle cells in zebrafish embryos under normal and dystrophic conditions. Clinical and experimental pharmacology & physiology. 39(1):78-86

Webb, S.E., Fluck, R.A., and Miller, A.L. (2011) Calcium signaling during the early development of medaka and zebrafish. Biochimie. 93(12):2112-25

Ulrich, F., Ma, L.H., Baker, R.G., and Torres-Vazquez, J. (2011) Neurovascular development in the embryonic zebrafish hindbrain. Developmental Biology. 357(1):134-51

Zhang, J., Webb, S.E., Ma, L.H., Chan, C.M., and Miller, A.L. (2011) Necessary role for intracellular Ca²⁺ transients in initiating the apical-basolateral thinning of enveloping layer cells during the early blastula period of zebrafish development. Development, growth & differentiation. 53(5):679-696

Webb, S.E., and Miller, A.L. (2011) Visualization of Ca2+ Signaling During Embryonic Skeletal Muscle Formation in Vertebrates. Cold Spring Harbor protocols. 3(2):a004325

Cheung, C.Y., Webb, S.E., Love, D.R., and Miller, A.L. (2011) Visualization, characterization and modulation of calcium signaling during the development of slow muscle cells in intact zebrafish embryos. The International journal of developmental biology. 55(2):153-74

Leung, C.F., Miller, A.L., Korzh, V., Chong, S.W., Sleptsova-Freidrich, I., and Webb, S.E. (2009) Visualization of stochastic Ca2+ signals in the formed somites during the early segmentation period in intact, normally developing zebrafish embryos. Development, growth & differentiation. 51(7):617-637

Mei, W., Lee, K.W., Marlow, F.L., Miller, A.L., and Mullins, M.C. (2009) hnRNP I is required to generate the Ca2+ signal that causes egg activation in zebrafish. Development (Cambridge, England). 136(17):3007-3017

Ma, L.H., Punnamoottil, B., Rinkwitz, S., and Baker, R. (2009) Mosaic hoxb4a neuronal pleiotropism in zebrafish caudal hindbrain. PLoS One. 4(6):e5944

Lam, P.Y., Webb, S.E., Leclerc, C., Moreau, M., and Miller, A.L. (2009) Inhibition of stored Ca release disrupts convergence-related cell movements in the lateral intermediate mesoderm resulting in abnormal positioning and morphology of the pronephric anlagen in intact zebrafish embryos. Development, growth & differentiation. 51(4):429-442

Ma, L.H., Webb, S.E., Chan, C.M., Zhang, J., and Miller, A.L. (2009) Establishment of a transitory dorsal-biased window of localized Ca(2+) signaling in the superficial epithelium following the mid-blastula transition in zebrafish embryos. Developmental Biology. 327(1):143-157

Cheung, Y.K. (2009) Exploring the role of Ca2+ signaling in slow muscle development using intact zebrafish embryos as an animal model. Ph.D. Thesis. :150p

Li, W.M., Webb, S.E., Chan, C.M., and Miller, A.L. (2008) Multiple roles of the furrow deepening Ca2+ transient during cytokinesis in zebrafish embryos. Developmental Biology. 316(2):228-248

Webb, S.E., Li, W.M., and Miller, A.L. (2008) Calcium signalling during the cleavage period of zebrafish development. Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 363(1495):1363-1369

Webb, S.E., and Miller, A.L. (2007) Ca(2+) Signalling and early embryonic patterning during zebrafish development. Clinical and experimental pharmacology & physiology. 34(9):897-904

Ma, L.H. (2007) Characterization of calcium signals during the blastula period of zebrafish (danio rerio) embryogenesis. Ph.D. Thesis. :239p

Montaville, P., Dai, Y., Cheung, C.Y., Giller, K., Becker, S., Michalak, M., Webb, S.E., Miller, A.L., and Krebs, J. (2006) Nuclear translocation of the calcium-binding protein ALG-2 induced by the RNA-binding protein RBM22. Biochimica et biophysica acta. Molecular cell research. 1763(11):1335-1343

Webb, S.E., and Miller, A.L. (2006) Ca(2+) signaling and early embryonic patterning during the Blastula and Gastrula Periods of Zebrafish and Xenopus development. Biochimica et biophysica acta. Molecular cell research. 1763(11):1192-1208

Li, W.M., Webb, S.E., Lee, K.W., and Miller, A.L. (2006) Recruitment and SNARE-mediated fusion of vesicles in furrow membrane remodeling during cytokinesis in zebrafish embryos. Experimental cell research. 312(17):3260-3275

Lee, K.W., Webb, S.E., and Miller, A.L. (2006) Requirement for a localized, IP 3 R-generated Ca 2+ transient during the furrow positioning process in zebrafish zygotes. Zygote (Cambridge, England). 14(2):143-155

Cheung, C.Y., Webb, S.E., Meng, A., and Miller, A.L. (2006) Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development. The International journal of developmental biology. 50(6):561-569

Cheung, Y.K. (2005) The development of alternative methods to introduce the Ca2+-sensitive bioluminescent complex, aequorin, into zebrafish embryos. Master's Thesis. :133p

Ma, O.L., Webb, S.E., and Miller, A.L. (2004) Imaging Patterns of Ca2+ Transients During the Blastula Period in Zebrafish Embryos. The Biological bulletin. 207(2):165

Miller, A.L., Leclerc, C., Moreau, M., Webb, S.E. (2004) Role of Ca2+ Signaling During Early Pronephric Development in Zebrafish and Xenopus Embryos. The Biological bulletin. 207(2):165

Cheng, J.C., Miller, A.L., and Webb, S.E. (2004) Organization and function of microfilaments during late epiboly in zebrafish embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 231(2):313-323

Ma, O.L., Webb, S.E., Miller, A.L. (2004) Imaging Patterns of Ca²⁺ Transients During the Blastula Period in Zebrafish Embryos.. The Biological bulletin. 207:165

Miller, A.L., Leclerc, C., Moreau, M., Webb, S.E. (2004) Role of Ca²⁺ Signaling During Early Pronephric Development in Zebrafish and Xenopus Embryos.. The Biological bulletin. 207:165

Lee, K.W., Ho, S.M., Wong, C.H., Webb, S.E., and Miller, A.L. (2004) Characterization of mid-spindle microtubules during furrow positioning in early cleavage period zebrafish embryos. Zygote (Cambridge, England). 12(3):221-230

Lee, K.W., Webb, S.E., and Miller, A.L. (2003) Ca2+ released via IP3 receptors is required for furrow deepening during cytokinesis in zebrafish embryos. The International journal of developmental biology. 47(6):411-421

Webb, S.E. and Miller, A.L. (2003) Calcium signalling during embryonic development. Nature reviews. Molecular cell biology. 4(7):539-551

Webb, S.E. and Miller, A.L. (2003) Imaging intercellular calcium waves during late epiboly in intact zebrafish embryos. Zygote (Cambridge, England). 11(2):175-182

Lee, K.W., Webb, S.E., Ho, S.M., Wong, C.H., and Miller, A.L. (2002) A microtubule array precedes the formation of the cleavage furrow during the first two-cell division cycles in zebrafish embryos. Developmental Biology. 247(2):465-466

Cheng, J.C., Miller, A.L., and Webb, S.E. (2002) Actin-based endocytosis contributes to zebrafish epiboly. The Journal of general physiology. 120(1):46

Cheng, J.C., Miller, A.L., and Webb, S.E. (2001) The role of microfilaments in zebrafish epiboly. The Journal of general physiology. 118(1):40

Leung, C.F., Webb, S.E., and Miller, A.L. (2000) On the mechanism of ooplasmic segregation in single-cell zebrafish embryos. Development, growth & differentiation. 42(1):29-40

Webb, S.E. and Miller, A.L. (2000) Calcium signalling during zebrafish embryonic development. BioEssays : news and reviews in molecular, cellular and developmental biology. 22(2):113-123

Lee, K.W., Webb, S.E., and Miller, A.L. (1999) A wave of free cytosolic calcium traverses zebrafish eggs on activation. Developmental Biology. 214(1):168-180

Pullium, J.K., Dillehay, D.L., and Webb, S. (1999) High mortality in zebrafish (Danio rerio). Contemp. Top. Lab. Anim. Sci.. 38(3):80-83

Gilland, E., Miller, A.L., Karplus, E., Baker, R., Webb, S.E. (1999) Imaging of multicellular large-scale rhythmic calcium waves during zebrafish gastrulation. Proceedings of the National Academy of Sciences of the United States of America. 96:157-161

Leung, C.F., S.E. Webb, and A.L. Miller (1998) Calcium transients accompany ooplasmic segregation in zebrafish embryos. Development, growth & differentiation. 40:313-326

Webb, S.E., K.W. Lee, E. Karplus, and A.L. Miller (1997) Localized calcium transients accompany furrow positioning, propagation, and deepening during the early cleavage period of zebrafish embryos. Developmental Biology. 192:78-92

Lee, K.W., Baker, R., Galione, A., Gilland, E.H., Hanlon, R.T., and Miller, A.L. (1996) Ionophore-induced calcium waves activate unfertilized zebrafish (Danio rerio) eggs. The Biological bulletin. 191(2):265-267