Figure 1

The interaction between SLC23A2 and ascorbic acid plays a role in the occurrence of cleft lip by altering oxidative stress-mediated apoptosis. Flowchart of this study.
Figure 2

Association analysis and pairwise LD block of the SNPs at chromosome 20. (A) Association analysis and linkage disequilibrium in the Chr20 region. (B,C) Pairwise LD block of the SNPs at chromosome 20 in the cases and controls with NSCLO represented by D’ and R2, respectively.
Figure 3

Construction of the SLC23A2-knockdown model in GMSM-K cells. (A) Immunofluorescence detection of the SLC23A2 protein in GMSM-K cells. (B) Transfected siRNA into GMSM-K cells. (C) Transfection efficiency was measured by qPCR. Error bars represent SD. n = 3; *, P < 0.05. Scale bar, 100 μm.
Figure 4

Changes in cell biology and ROS level in GMSM-K cells after SLC23A2 knockdown. (A,B) ROS levels after knockdown of SLC23A2. (C,D) Cell proliferation after knockdown of SLC23A2. (E,F) Cell apoptosis after knockdown of SLC23A2. (G,H) Change in cell migration after SLC23A2 knockdown. (I) Volcano plot for differential gene expression. (J) KEGG enrichment analysis of DEGs. (K). The heat map of all DEGs, with genes related to cell proliferation, cell apoptosis, and cell cycle regulation given particular attention. (L) RT-qPCR verification result for the DEGs. Error bars represent SD. n = 3; ns, P > 0.05; **, P < 0.01; ***, P < 0.001.
Figure 5

Effect of the SLC23A2/exogenous ROS/AA interaction on cellular oxidative stress level and cellular biology in GMSM-K cells. (A,B). Effect of the SLC23A2/exogenous ROS/AA interaction on ROS levels. (C) Effect of the SLC23A2/exogenous ROS/AA interaction on GSH/GSSG. (D) Effect of the SLC23A2/exogenous ROS/AA interaction on SOD activity. (E) Effect of the SLC23A2/exogenous ROS/AA interaction on cell apoptosis. Error bars represent SD. n = 3; ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Scale bar, 100 μm.
Figure 6

Effect of the slc23a2/exogenous ROS/AA interaction on oxidative stress and apoptosis in zebrafish cells. (A) Effect of the slc23a2/exogenous ROS/AA interaction on ROS levels. (B) Effect of the slc23a2/exogenous ROS/AA interaction on apoptosis. slc23a2-MO, slc23a2-knockdown zebrafish cells. Error bars represent SD. n = 3; ns, P > 0.05; ****, P < 0.0001.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Pediatr
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.