Effects of lhx4 knockdown via vivo-MO in the eyes on the apoptosis in the retina. All figures are horizontal sections along the temporal-nasal axis (T-N). (A–F) Immunofluorescence staining with Caspase3 at 60 hpf and 72 hpf. Blue, DAPI staining of the nuclei. Scale bar = 20 μm. (G,H) Statistical analysis of the number of Caspase3⁺ cells in WT, vCT, and 4vMO retinas at 60 hpf and 72 hpf. ns, P > 0.05; vCT vs. WT; 4vMO vs. vCT. Results are presented as the mean ± SEM (n ≥ 10).