Figure 1

SEM images (A,B), size distribution (C), and zeta potential (D) of BC.
Figure 2

Hepatic H&E-stained sections of zebrafish. Control (A); 1 μg/L MC-LR (B); 5 μg/L MC-LR (C); 25 μg/L MC-LR (D); 100 μg/L BC (E); 1 μg/L MC-LR+ BC (F); 5 μg/L MC-LR + BC (G); and 25 μg/L MC-LR + BC (H). Pyknotic nuclei (black arrow), swollen hepatocytes (white arrowhead), and vacuolization (black arrowhead). Bar = 50 um.
Figure 3

Hepatic ultrastructural alterations in zebrafish. Control (A); 1 μg/L MC-LR (B); 5 μg/L MC-LR (C); 25 μg/L MC-LR (D); 100 μg/L BC (E); 1 μg/L MC-LR + BC (F); 5 μg/L MC-LR+ BC (G); and 25 μg/L MC-LR + BC (H). Swollen mitochondria (black arrowhead); deformed nuclei (white arrowhead); vacuolization (white arrow); and dilated endoplasmic reticulum (black box). Mt, mitochondria; Nu, nuclei; ER, endoplasmic reticulum. Bar = 1 μm.
Figure 4

Changes in MDA content (A), SOD activity (B), CAT activity (C), GPx activity (D), GST activity (E), and GSH content (F) in the liver of zebrafish. Asterisks (* p < 0.05; ** p < 0.01) indicate significant differences between the treatments and control. Hashes (# p < 0.05) indicate significant differences between the MC-LR + BC groups and the MC-LR-only groups, respectively. The values are presented as mean ± SD (n = 3).
Figure 5

Heatmap of Nrf2 signaling pathway gene expression. Asterisks (* p < 0.05; ** p < 0.01) indicate significant differences between the treatments and control. The dotted box with hashes (# p < 0.05; ## p < 0.01) indicate significant differences between the MC-LR + BC groups and the MC-LR-only groups, respectively. The values are presented as mean ± SD (n = 6).
Acknowledgments
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