FIGURE SUMMARY
Title

Preparation of Baicalin Liposomes Using Microfluidic Technology and Evaluation of Their Antitumor Activity by a Zebrafish Model

Authors
Gu, Y., Jin, L., Wang, L., Ma, X., Tian, M., Sohail, A., Wang, J., Wang, D.
Source
Full text @ ACS Omega
Fig. 1

Graphic presentation of the BCL-LPs preparation process by chip.

Fig. 2

Effect of total flow rates and flow rate ratios on the liposome particle size and PDI.

Fig. 3

Effect of a single factor on the size, PDI, and EE of BCL-LPs. a and b: effects of phospholipids concentration; c and d: effects of phospholipids to cholesterol ratio; e and f: effects of tween-80 concentration. n = 5 for each group.

Fig. 4

3D response surface plots and 2D contour plots showing the effects of various parameters on EE.

Fig. 5

Characterization of the BCL-LPs. a: TEM images of BCL-LPs, b: FT-IR analysis of BCL, LPs, and BCL-LPs.

Fig. 6

In vitro release simulations of BCL-LPs. n = 5 for each group

Fig. 7

Effects of pH on particle size (a), PDI (b), zeta potential (c), and appearance (d). Effects of ionic strength on particle size (a), PDI (b), zeta potential (c), and appearance (d). n = 5 for each group.

Fig. 8

Thermal stability of the BCL-LPs. n = 5 for each group.

Fig. 9

Storage stability of BCL-LPs at 4 and 25 °C for 30 days. a, b and c with the effects on particle size, polydispersity index, and zeta potential. n = 5 for each group.

Fig. 10

Treatment of zebrafish larvae with different concentrations of tested compounds induced an obvious fluorescence enhancement in the zebrafish liver. Scale bar is 200 μm. A: treated with Doxc (20 μg/mL), BCL-LPs (0.5, 1, 2, 4, and 8 μg/mL), B: treated with BCL (0.5, 1, 2, 4, and 8 μg/mL).

Fig. 11

Fluorescence intensity in zebrafish liver after incubation with tested compounds and Doxc. Groups: Control (Ctl), BCL-LPs, BCL, Doxycycline Hyclate (Doxc). The data were analyzed by one-way ANOVA followed by Dunett’s test using Graph Pad Prism 7.0 (GraphPad Software; CA, USA). n = 5 for each group. The results were expressed as mean ± SEM, *p < 0.05, **p < 0.01 VS Ctl.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ ACS Omega
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