FIGURE SUMMARY
Title

Analysis of the morphology of retinal vascular cells in zebrafish (Danio rerio)

Authors
Middel, C.S., Dietrich, N., Hammes, H.P., Kroll, J.
Source
Full text @ Front Cell Dev Biol
FIGURE 1

Identification of endothelial cells using the Tg(nflk:EGFP) zebrafish line. (A): Image of the zebrafish retina at ×200 magnification after hemalum stain taken with a light microscope. Scale bar: 100 µm. (B): Image of the same zebrafish retina taken with a confocal microscope directly after digestion.
FIGURE 2

Trypsin digest of the zebrafish retina. ×200 magnification of a zebrafish retinal trypsin digest preparation. Black arrow: Erythrocyte. White arrow: Endothelial cell. Red arrow: vascular mural cell. Scale bar: 100 µm.
FIGURE 3

Analysis of the adult zebrafish retina according to density areas and distance to the entrance of the optic artery into the retina. (A) Overview image of a retinal trypsin digest preparation, ×40 magnification. Scale bar: 100 µm. (B) Schematic overview of the areas analysed. (C) Representative images of the different parts of the retina (central, intermediate, peripheral), ×200 magnification. Scale bar: 100 µm. This figure was in part created with BioRender.com.
FIGURE 4

The vessel diameter varies between the different areas in the intermediate part of the retina. (A–C) Vessel diameter [µm] in the different parts of the retina according to density areas. Abbreviations: LD, low density area; MD1/2, medium density area 1/2; HD, high density area.
FIGURE 5

The number of endothelial cells and mural cells per mm2 does not vary between the different areas of the retina when calculated in the correlating parts. (A–C) Number of endothelial cells per mm2 in the central, intermediate and peripheral parts of the retina according to density area. (D–F) Number of mural cells per mm2 in the central, intermediate and peripheral parts of the retina according to density area. Abbreviations: EC, endothelial cell; vMC, vascular mural cell; LD, low density area; MD1/2, medium density area 1/2; HD, high density area. n = 6 zebrafish eyes. Central area: 1 image/density area/eye, intermediate area: 2 images/density area/eye, peripheral area: 3 images/density area/eye.
FIGURE 6

Zebrafish have higher absolute numbers of endothelial cells and vascular mural cells than mammals and a higher vascular mural cell to endothelial cell ratio. Abbreviations: EC, endothelial cells; P, pericytes (mouse/rat/human); vMC, vascular mural cells (zebrafish). The data from mouse, rat and human were originally described in (Hammes, 2018).
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol
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