FIGURE SUMMARY
Title

Effects of Zinc Oxide Nanoparticle Exposure on Human Glial Cells and Zebrafish Embryos

Authors
Valdiglesias, V., Alba-González, A., Fernández-Bertólez, N., Touzani, A., Ramos-Pan, L., Reis, A.T., Moreda-Piñeiro, J., Yáñez, J., Laffon, B., Folgueira, M.
Source
Full text @ Int. J. Mol. Sci.
Figure 1

Analysis of Zn2+ ions released from ZnO NPs in cell culture medium and dechlorinated water. Bars represent the standard deviation.
Figure 2

Morphology of A172 glial cells subjected to different treatments: control (A), treated with ZnO NPs at 10 µg/mL (B), 50 µg/mL (C), and 100 µg/mL (D) and treated with ZnSO4 at 0.1 mM (E) and 0.2 mM (F). Scale bar: 50 µm.
Figure 3

Cytotoxicity of ZnO NPs in A172 glial cells at different exposure times. PC: positive control. Bars represent mean ± standard error. * p < 0.05, significant difference regarding the corresponding control.
Figure 4

Cytotoxicity of ZnSO4 in A172 glial cells at different exposure times. PC: positive control. Bars represent mean ± standard error. ** p < 0.01, significant difference regarding the corresponding control.
Figure 5

Survival and hatching in zebrafish embryos exposed to ZnO NPs for 96 h. * p < 0.05; significant difference regarding the corresponding control.
Figure 6

Survival and hatching in zebrafish embryos exposed to ZnSO4 for 96 h. * p < 0.05; ** p < 0.01, significant difference regarding the corresponding control.
Figure 7

Analysis of zebrafish embryos morphology after exposure to ZnO NPs and ZnSO4. (a) Images showing the general morphology of zebrafish embryos after 96 h exposure to dechlorinated water only (control; top), ZnO NPs (100 µg/mL, middle) and ZnSO4 (0.08 mM; bottom). Selected individuals are representative of each group. Scale bar 500 µm. (b) Body length and (c) normalized eye size. Bars represent mean ± standard error.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.
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