FIGURE SUMMARY
Title

Development of a Transparent Transgenic Zebrafish Cellular Phenotype Tg(6xNF-kB:EGFP); Casper(roy-/-, nacre-/-) to Study NF-kB Activity

Authors
Rajpurohit, S.K., Ouellette, L., Sura, S., Appiah, C., O'Keefe, A., McCarthy, K., Kandepu, U., Ye Mon, M., Kimmerling, K., Arora, V., Lokeshwar, B.L.
Source
Full text @ Biomedicines
Figure 1

The zebrafish phenotype expression used in this experiment is Casper(roy−/−, nacre−/−) (top row) and the WT-AB phenotype (bottom row). An example of each sex is present for each phenotype, the females for each line (left column) and the males (right column). The inflammatory transgenic Tg(NF-kB:GFP) has a normal wild-type skin pigmentation pattern.
Figure 2

Cross-breeding of Casper and NF-kB:EGFP zebrafish progeny. The green dot represents the presence of NF-kb:GFP. The experiment is to develop a transparent transgenic zebrafish that expresses both the Casper phenotype and the NF-kB genotype.
Figure 3

In vivo imaging of the 72 hpf (hours post-fertilization) stage of the newly developed zebrafish strain of Tg(NF-kB:EGFP); Casper(roy−/−, nacre−/−). Green fluorescent protein (EGFP) shows NF-kB specific to inflammatory protein and Casper transparent skin mutant in which they represent deleted melanophore and xanthophore genes that make the transparent skin.
Figure 4

Transgenic expression in newly generated zebrafish cellular phenotype of Tg(NF-kB:EGFP); Casper(roy−/−, nacre−/−) (percentage of population).
Figure 5

Percentile of skin pigmentation background pattern of newly generated NF-kB:EGFP zebrafish phenotype, the Casper(roy−/−, nacre−/−), the nacre mutant, the roy orbison mutant, and the WT-AB normal skin pigmentation pattern at the adult stage (percentage of population).
Figure 6

The green fluorescent protein (EGFP) shows NF-kB specific expression and the Casper transparent skin mutant in which they represent deleted melanophore and xanthophore genes that make the transparent skin. (A) Embryonic stage at 42 hpf (hours post-fertilization), (B) larval stage at 96 hpf (hours post-fertilization), and (C1,C2) head region of the adult stage, (C1): 1.5× image and (C2): 7× image.
Figure 7

Confocal in vivo imaging of the newly developed zebrafish strain Tg(NF-kB:EGFP); Casper(roy−/−, nacre−/−). The green fluorescent protein (EGFP) shows NF-kB specific expression and the Casper transparent skin mutant in which they represent deleted melanophore and xanthophore genes that make the transparent skin. (A1–A3) Larval stage at 72 hpf (hours post-fertilization), (B1–B3) larval stage at 96 hpf (hours post-fertilization), and (C1,C2) head region of the adult stage.
Figure 8

Flow cytometry Analysis: The inflammatory impact of LPS treatment and the inhibitory effect of K21 drug in fish larvae of Casper mutant (blue bar) as control and GFP expression in the transparent transgenic Casper/NF-kB:GFP strain (green bar). The GFP expression of flow cytometry data expressed in percentile (%).
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomedicines
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