FIGURE SUMMARY
Title

DNAJA4 Promotes the Replication of the Chinese Giant Salamander Iridovirus

Authors
Liu, Z., Xie, D., He, X., Zhou, T., Li, W.
Source
Full text @ Genes (Basel)
Figure 1

Amino acid alignment of DNAJA4s from different species. The conserved domains (aa 0–70), respectively. The accession numbers were listed as follows: Epinephelus coioides, MN937271; Danio rerio, XP_002666797.3; Stegastes partitus, XP_008275021.1; Gallus gallus, XP_413746.5; Chrysemys picta bellii, XP_005294547.1; Homo sapiens, AIC63476.1; Mus musculus, AAH22948.1; Xenopus tropicalis.
Figure 2

Phylogenetic analysis of DNAJA4 homologs from different species. A phylogenetic tree was constructed based on the protein sequences of DNAJA4-like genes from different species using MEGA 7.0 with the neighbor-joining (NJ) method. Numbers at the nodes denote the bootstrap values of 1000 replicates. Scale represents the numbers of substitutions per 1000 bases.
Figure 3

Viral infection affected the expression and localization of EPC DNAJA4. (A) Effect of viral infection on EPC DNAJA4 transcription levels; (B) effects of viral infection on the localization of EPC DNAJA4. The results were expressed as mean ± SD, (n = 3; * p < 0.05; *** p < 0.001; **** p < 0.0001).
Figure 4

Effect of overexpression of DNAJA4 on CGSIV replication: (A) transcriptional level detection 24 h after overexpression of pcDNA3.1-DNAJA4-HA plasmid; (B) detection of protein level of plasmid overexpressing pcDNA3.1-DNAJA4-HA; (C) effect of overexpression of DNAJA4 for 48 h on viral titer; and (D) effect of overexpression of DNAJA4 on the copy number of viral genomic DNA. The results were expressed as mean ± SD, (n = 3; * p < 0.05; **** p < 0.0001).
Figure 5

Knockdown of DNAJA4 by siRNA attenuates CGSIV replication and CGSIV DNA replication: (A) transfection efficiency of fluorescent siRNA; (B) qPCR detection of siRNA interference effect; (C) results of siRNA interference with CPE; (D) effect of siRNA interference on the titer of CGSIV virus; and (E) effect of siRNA interference on genome replication of CGSIV virus. The results were expressed as mean ± SD, (n = 3; * p < 0.05; ** p < 0.01).
Figure 6

Interaction between EPC DNAJA4 and CGSIV PCNA: (A) Co-IP detects the interaction between EPC DNAJA4 and CGSIV PCNA. CO-IP was immunoblotted with anti-Flag antibody and anti-HA antibody. (B) His–pulldown and GST–pulldown verified that EPC DNAJA4 interacts with CGSIV PCNA. (C) Immunofluorescence detection shown of DNAJA4-HA and PCNA-Flag co-localization in EPC.
Acknowledgments
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