FIGURE SUMMARY
Title

Toxicology evaluation of overdose hydroxychloroquine on zebrafish (Danio rerio) embryos

Authors
Luo, M., Xie, D., Lin, Z., Sun, H., Liu, Y.
Source
Full text @ Sci. Rep.
Figure 1

Survival curves of zebrafish embryos exposed to different concentrations of HCQ from 8 hpf to 4 dpf. Lines of different colors represent different groups, and green represents the control group. (A) Unpaired t test was used to test for statistical significance *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, the same as below. (B–E) Images are examples of developmental malformations. Scale bars, 100 μM.
Figure 2

(A) Scatter plot and statistical results of the number of coiling tail at 1 dpf. (B) Scatter plot and statistical results of hatching rate in 3 dpf. (C) Scatterplot and statistical results of heart rate in 4 dpf. (D) Scatter plot and statistical results of body length in 5 dpf.
Figure 3

Germ layer marker expression detected by ISH during gastrulation at 5 hpf (A) and 7 hpf (B). Orientation: ntla and sox17, dorsal views with animal pole to the top at 75% epiboly stage; dorsal-animal views with anterior towards the top: otx2; others, animal-pole view (eve1, chd, gsc and gata2a) with dorsal to the right. The scatter plot on the right corresponds to the statistical results of hybridization signals. Scale bars, 100 μM.
Figure 4

(A) Marker expression at 24 hpf. Lateral views with anterior to the left. (B) Heart morphology (marked by the pan-cardiomyocyte marker myl7) at 48 hpf. Embryo orientation: ventral view with the anterior at the top. Arrows point to the atrium; red lines demonstrate the acute angle formed between the atrial and ventricular axes. The scatter plot on the right corresponds to the statistical results of angles.
Figure 5

(A) Overall statistics of differential genes. (B) Significantly differential genes associated with the development of the cardiovascular system. (C) Significantly differential genes associated with eye and optic nerve development. (D) Number of significantly differentially expressed genes for biological processes, cellular components, and molecular functions. (E) Significantly differential genes associated with the development of the nervous system.
Figure 6

The top 20 enriched KEGG pathways, the pathway selected by the red dashed box is the only one significantly enriched.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.
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