FIGURE SUMMARY
Title

Golgi-Targeting Fluorescent Probe for Monitoring CO-Releasing Molecule-3 In Vitro and In Vivo

Authors
Li, S., Yang, K., Zeng, J., Ding, Y., Cheng, D., He, L.
Source
Full text @ ACS Omega
Fig. 1

Fluorescence spectra of Golgi-Nap-CORM-3 (10 μM) upon incubation with a series of concentrations of CORM-3 (0, 0.5, 1, 5, 10, 20, 25, 35, 40, 50, 60, 70, and 80 μM, 37 °C) for 60 min. Inset: the curve was plotted with the probe fluorescence intensity (FI) vs CORM-3 concentration. λex = 430 nm.
Fig. 2

Frontier orbital diagrams of (A) compounds Golgi-Nap-CORM-3 and Golgi-Nap-NH2 and (B) separate naphthalimide, nitro, and amino units.
Fig. 3

Selectivity of Golgi-Nap-CORM-3 (10 μM) toward various analytes (100 μM, except 500 μM Cys, Hcy and 1 mM GSH). (1, Blank; 2, CORM-3; 3, H2O2; 4, •OH; 5, 1O2; 6, ClO–; 7, O2•–; 8, ONOO–; 9, NO; 10, GSH; 11, Cys; 12, Hcy; 13, H2S; 14, Vc; 15, Fe2+; 16, Fe3+; 17, Cu2+; 18, Zn2+; 19, HCO3–; 20, CO32–; and 21, SO42–.) λex = 430 nm.
Fig. 4

Time-dependent intensity of Golgi-Nap-CORM-3 (10 μM) at 520 nm after addition of concentrations of CORM-3 (0, 20, 40, 60, and 80 μM). λex = 430 nm.
Fig. 5

Colocalization fluorescence images of HeLa cells coincubated with Golgi-Nap-CORM-3 (10 μM), CORM-3 (50 μM), and Golgi Tracker Red (500 nM). (a) HeLa cells incubated with CORM-3 for 1 h, followed by treatment with Golgi-Nap-CORM-3 for 1 h in the green channel, λex = 458 nm, collected at 481–580 nm. (b) HeLa cells stained with Golgi Tracker Red (500 nM) for 0.5 h in the red channel, λex = 561 nm, collected at 580–620 nm. (c) Merged image of (a) and (b). (d) Bright-field image. (e) Merged image of (a)–(d). (f) Intensity scatter plot of the green channel and the red channel. Scale bars are 20 μm.
Fig. 6

Confocal fluorescence images of HeLa cells. (a–e) Cells incubated with different concentrations of CORM-3 (0, 20, 40, 60, and 80 μM) for 1 h, followed by treatment with 10 μM Golgi-Nap-CORM-3 for 1 h. (f) Relative FI of (a)–(e). λex = 458 nm, collected at 481–580 nm. Scale bar: 20 μm.
Fig. 7

Confocal fluorescence images of zebrafish. (a) Zebrafish treatment with 10 μM Golgi-Nap-CORM-3 for 1 h. (b) Zebrafish incubated with 80 μM CORM-3 for 1 h, followed by treatment with 10 μM Golgi-Nap-CORM-3 for 1 h. (c) Relative FI of (a) and (b). λex = 458 nm, collected at 481–580 nm.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ ACS Omega
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