a The structure of NemR^C106 (PDB ID: 4YZE) shows the N-terminal DNA-binding domain (colored blue), the C-terminal sensory domain (colored cyan), Cys106 and Lys175 (colored yellow), and the flexible loop (colored red) into which cpYFP was inserted. The N- and C-termini are indicated with N and C, respectively. b The proposed simplified scheme of NemR-cpYFP biosensors functioning in living cells. c The structure of Hypocrates is presented with NemR^C106 colored blue/cyan, cpYFP colored yellow, the linkers between NemR^C106 and cpYFP colored green, and the flexible loop colored red. The upper numbers represent amino acid numbering corresponding to the intact NemR^C106, while the lower numbers represent numbering corresponding to the biosensor. d Optical properties of purified Hypocrates protein in PBS. e Hypocrates fluorescence excitation spectra in E. coli cells in reduced and NaOCl-oxidized forms. f Purified Hypocrates (0.5 µM) fluorescence excitation spectrum behavior in the presence of NaOCl in saturating concentration. g Purified Hypocrates (0.5 µM) fluorescence excitation spectrum behavior in the presence of N-chlorotaurine (NCT) in saturating concentration. h HypocratesCS fluorescence excitation spectra in E. coli cells in reduced and NaOCl-oxidized forms. i HypocratesKA fluorescence excitation spectra in E. coli cells in reduced and NaOCl-oxidized forms. Source data are provided as a Source Data file.

a Far-UV circular dichroism spectra of reduced and NaOCl oxidized Hypocrates. With increasing NaOCl concentration, an increase of the molar ellipticity [θ] at 208 nm and 222 nm and a decrease at 194 nm were observed. b Upon reduction with DTT, the NaOCl-treated biosensor restores its overall secondary structure to the reduced form. c The excitation ratio of reduced and oxidized Hypocrates depends on the pH value of the buffer solution. NCT – N-chlorotaurine. The data are presented as a mean ± SEM, n = 4 for reduced Hypocrates, n = 3 for other curves. d Selectivity profile of purified Hypocrates (2 µM) towards a set of various redox compounds. ROS - reactive oxygen species, RNS - reactive nitrogen species, RES - reactive electrophilic species, XOX - xanthine oxidase, X - xanthine, CAT - catalase. The data are presented as the mean ± SEM, n = 8 for NaOCl, n = 5 for NCT, n = 6 for ONOO⁻, n = 3 for other compounds. Source data are provided as a Source Data file.

Changes in the fluorescence excitation spectra of Hypocrates (0.5 µM) obtained by additions of a NaOCl or b N-chlorotaurine (NCT) aliquots. c Titration curves of Hypocrates (0.5 µM) in sodium phosphate buffer obtained by additions of NaOCl, NaOBr, HOSCN, or NCT aliquots. The data are presented as a mean ± SEM (for n > 2), n ≥ 2. The maximum amplitudes of response are 2.0-, 1.8-, 1.8- and 1.7-fold for NaOBr, HOSCN, NaOCl, and NCT, respectively. In the presence of NaOBr, NaOCl, and NCT, the probe is saturated at ~5 µM, and for HOSCN at ~1 µM. d The same data as in c, Hypocrates sensitivity towards NaOCl, NaOBr, NCT, and HOSCN is shown. The data are presented as a mean ± SEM (for n > 2), n ≥ 2. e–g Hypocrates reaction rates. Changes in cpYFP fluorescence at > 515 nm cut-off (λ_ex = 485 nm) were measured as a function of time (insert). The curves were fitted to a double exponential to obtain the observed rate constants (k_obs/fast), which were plotted as a function of different e NaOCl, f NCT, or g NaOBr concentrations. The second-order rate constants for NaOCl (1.4 ± 0.056) × 10⁶ M⁻¹s⁻¹, NCT (6.1 ± 0.3) × 10⁴ M⁻¹s⁻¹, and NaOBr (4.5 ± 0.25) × 10⁶ M⁻¹s⁻¹ were determined from the slope of the straight line [k_fast = k_on⋅[oxidant] + k_off]. The data are presented as a mean ± SD (for n > 2), n ≥ 2. Source data are provided as a Source Data file.

a The NemR-sensor domain (green) and the cpYFP domain (yellow) are shown. The chromophore (CR2) in the cpYFP β-barrel is shown in orange stick representation. S355 (to which the reactive C355 was mutated to) and K424 (two orientations of the side chain) are shown in red stick representation. The linkers “SAG” and “GT” are colored blue. The N-terminal residues 1–7 and segments (residues 40-42 and 193–207) are missing. b Superposition of NemR^C106 (blue – PDB ID: 4YZE) with the NemR-sensor domain (green). RMSD - root-mean-square deviation. c N95 connects the sensor domain (green) with cpYFP (yellow). N95 interacts with the backbone of Q96, F97 of the NemR-sensor domain and with S166 of the cpYFP domain. The 4-hydoxybenzyl group of CR2 interacts with the phenyl-ring of F164 over a distance of 3.8 Å. d The imidazolinone ring of CR2 interacts with R303, Q301, V268, E183, and T269.

a Fluorescence excitation spectra of purified Hypocrates (0.5 µM) in the presence of individual myeloperoxidase (MPO), H₂O₂ and the MPO-H₂O₂ system. b Hypocrates (0.5 µM) signal as a function of time in the presence of individual H₂O₂ and the MPO-H₂O₂ system. HOCl, generated by MPO, leads to the development of a saturating response, while a physiologically irrelevant H₂O₂ concentration induces only minor signal changes. c The titration curve of Hypocrates in HeLa Kyoto cells exposed to different concentrations of NaOCl (values ± SEM, N = 2 experiments, n ≥ 25 cells per experiment). d Upper part: Hypocrates fluorescence changes induced by 17 nmol NaOCl/(10⁵ cells) (values ± SEM, N = 2 experiments, n ≥ 30 cells per experiment). Lower part: Images of Hypocrates in transiently transfected HeLa Kyoto cells exposed to 17 nmol/(10⁵ cells) NaOCl at different time points. Scale bar = 50 µm. The lookup table indicates changes in the Ex₅₀₀/Ex₄₂₅ ratio. e Upper part: Images of human neutrophils phagocytosing E. coli cells that express Hypocrates or the control version HypocratesCS. Scale bar = 10 µm. The lookup table indicates changes in the Ex₅₀₀/Ex₄₂₅ ratio. Lower part: Hypocrates (blue line) and HypocratesCS (red line) fluorescence changes in E. coli cells phagocytosed by human neutrophils. The starting point on the graph corresponds to the moment at which individual bacteria are phagocytosed by a neutrophil (values ± SEM, N = 3 experiments, 35 bacterial cells in total for each version of the sensor). Source data are provided as a Source Data file.

a Hypocrates and HyPerRed imaging. Zebrafish embryos were co-injected with Hypocrates or HypocratesCS and HyPerRed mRNAs at the 1-cell stage, and a tail fin amputation assay was performed on 48 h post-fertilization larvae. Images were taken before amputation, and time lapse imaging was performed up to 60 min post-amputation (mpa). The lookup tables indicate changes in the oxidation states of the sensors. Scale bar = 100 µm. b Hypocrates ratio and HyPerRed fluorescence were quantified at the amputation plane and normalized to the mean fluorescence on the uncut tail for each larva. Ratio quantification on larvae tail fin expressing Hypocrates (blue lines) or HypocratesCS (red lines) is shown. Two-way ANOVA test with a Tukey’s multiple comparisons posttest was used to determine if the observed difference was statistically significant. Non-amputated embryos (dashed lines) expressing Hypocrates or HypocratesCS were also imaged as a control (values ± SEM; N = 4 experiments, n ≥ 3 embryos/timepoint; ns no significant, **P < 0.01, ***P < 0,001, versus HypocratesCS cut larvae, P-values are shown). For control embryos (dotted lines), fluorescence has been normalized for each embryo to the first image of the time lapse (t = 0). HyPerRed fluorescence quantification on larvae tail fin expressing HyPerRed (black lines) is shown (values ± SEM, N = 3 experiments, n ≥ 7 embryos/timepoint; ***P < 0.001, versus uncut larvae (dashed line), P-values are shown). Source data are provided as a Source Data file.