Fig. 1

Cumulative survival rate (%) of zebrafish after challenge with Vibrio spp.

Fig. 2

The Serum immune (a), hematological responses (b), and growth parameter (c &d) enrichment of zebrafish fed with different diet.

Fig. 3

The effect of singular and combination feed on the relative expression of IL-beta gene. P < 0.05 was considered as statistically significant and it is presented as ns (P > 0.05), ∗(P ≤ 0.05), ∗∗(P ≤ 0.01), ∗∗∗(P ≤ 0.001) ∗∗∗∗(P ≤ 0.0001).

Fig. 4

The effect of singular and combination feed on the relative expression of TNF – alpha gene. P < 0.05 was considered as statistically significant and it is presented as ns (P > 0.05), ∗(P ≤ 0.05), ∗∗(P ≤ 0.01), ∗∗∗(P ≤ 0.001) ∗∗∗∗(P ≤ 0.0001).

Fig. 5

The effect of singular and combination feed on the relative expression of lysozyme gene. P < 0.05 was considered as statistically significant and it is presented as ns (P > 0.05), ∗(P ≤ 0.05), ∗∗(P ≤ 0.01), ∗∗∗(P ≤ 0.001) ∗∗∗∗(P ≤ 0.0001).

Fig. 6

The effect of singular and combination feed on the relative expression of SOD gene. P < 0.05 was considered as statistically significant and it is presented as ns (P > 0.05), ∗(P ≤ 0.05), ∗∗(P ≤ 0.01), ∗∗∗(P ≤ 0.001) ∗∗∗∗(P ≤ 0.0001).

Acknowledgments
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