FIGURE SUMMARY
Title

Hesperidin Interacts With CREB-BDNF Signaling Pathway to Suppress Pentylenetetrazole-Induced Convulsions in Zebrafish

Authors
Sharma, P., Kumari, S., Sharma, J., Purohit, R., Singh, D.
Source
Full text @ Front Pharmacol
FIGURE 1

Docking results obtained from LeadIT for the four receptors with hesperidin. (A) 3D and 2D interaction diagram of Hesperidin with NMDAR receptor. (B) 3D and 2D interaction diagram of Hesperidin with TRK receptor. (C) 3D and 2D interaction diagram of Hesperidin with GABA receptor. (D) 3D and 2D interaction diagram of Hesperidin with Interleukin-10 receptor; (i) 3D pose, and (ii) 2D pose.
FIGURE 2

Snapshots of the complexes at 0 ns (start), 25 ns (midway), and 50 ns (end) of the simulation visualised in Discovery studio. (A) NMDAR-Hesperidin, (B) TRK-Hesperidin, (C) GABA-Hesperidin, and (D) Interleukin10-Hesperidin.
FIGURE 3

Effect of hesperidin on PTZ-induced hyperactivity in 7 dpf zebrafish larvae. (A) Mean swimming speed, (B) total distance travelled, and (C) larva movement track. aP < 0.05 as compared to veh/naive (naïve) group and; bP < 0.05 as compared to veh/ptz (vehicle control) group. The results expressed as mean ± SEM. veh/naive: system water incubated group not exposed to PTZ; veh/ptz: system water incubated group exposed to PTZ; hes1/ptz: Hesperidin 1 μM incubated group exposed to PTZ; hes5/ptz: Hesperidin 5 μM incubated group exposed to PTZ and; hes10/ptz: Hesperidin 10 μM incubated group exposed to PTZ.

FIGURE 4

Effect of hesperidin on the latency to PTZ-induced seizures (Stage 3). veh/naïve group was not exposed to PTZ, hence not shown in the figure. aP < 0.05 as compared to veh/ptz (vehicle control) group; bP < 0.05 as compared to hes1/ptz group and; cP < 0.05 as compared to hes5/ptz group. The results expressed as mean ± SEM. veh/ptz: system water incubated group exposed to PTZ; hes1/ptz: Hesperidin 1 μM incubated group exposed to PTZ; hes5/ptz: Hesperidin 5 μM incubated group exposed to PTZ and; hes10/ptz: Hesperidin 10 μM incubated group exposed to PTZ.
FIGURE 5

Effect of hesperidin on PTZ-mediated alterations in mRNA levels of (A)c-fos, (B)bdnf, and (C)il-10. aP < 0.05 as compared to veh/naive-s (naive) group and; bP < 0.05 as compared to veh/ptz-s (vehicle control). The results expressed as mean ± SD. veh/naïve-s: system water incubated group not exposed to PTZ; veh/ptz-s: system water incubated group exposed to PTZ; and; hes10/ptz-s: Hesperidin 10 μM incubated group exposed to PTZ.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Pharmacol
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