The toxicity of thyme oils in zebrafish larvae (a). Mortality curves until 48 hpf (b). Results are presented as means ± SD (n = 15). YSE: yolk sac edema; PE: pericardial edema; CBS: curved body shape.

Thyme oils inhibited MTZ-induced oxidative insults in skin cells of Tg (krt4: NTR-hKikGR)^cy17 transgenic zebrafish (a): Control group (I); MTZ-treatment group (II); MTZ + L-NAC-treatment gruop (III); MTZ + YL-thyme oil-treatment group at the concentration of 5 μg/mL (IV), 10 μg/mL (V) and 20 μg/mL (VI); MTZ + JB-thyme oil-treatment group at the concentration of 5 μg/mL (VII), 10 μg/mL (VIII) and 20 μg/mL (IX); MTZ + QY-thyme oil-treatment group at the concentration of 5 μg/mL (X), 10 μg/mL (XI) and 20 μg/mL (XII); MTZ + LD-thyme oil-treatment group at the concentration of 5 μg/mL (XIII), 10 μg/mL (XIV) and 20 μg/mL (XV); Determination of fluorescence intensity by Image analysis (b); The values are expressed as mean ± SD (n = 15). * Represents p-value less than 0.05 and ** represents p-value less than 0.01 vs. MTZ group; # represents p value less than 0.05 and ## represents p value less than 0.01 vs. control group.

Determination of ROS levels in zebrafish larvae from 48 hpf in Control group, AAPH-treatment group, AAPH + L-NAC-treatment group (a); Determination of ROS levels in zebrafish larvae from 48 hpf after treatment with YL-thyme oil, JB-thyme oil, QY-thyme oil and LD-thyme oil (b); Determination of mean DCF fluorescence intensity by Image analysis (c). The values are expressed as mean ± SD (n = 15). * Represents p-value less than 0.05 and ** represents p value less than 0.01 vs. AAPH-induced group; # represents p-value less than 0.05 and ## represents p-value less than 0.01 vs. control group.