FIGURE SUMMARY
Title

Nutritional programming improves dietary plant protein utilization in zebrafish Danio rerio

Authors
Kwasek, K., Wojno, M., Iannini, F., McCracken, V.J., Molinari, G.S., Terova, G.
Source
Full text @ PLoS One

Dietary treatment regimens tested in the study.

NP–nutritional programming, FM–fishmeal, PP–plant protein, SBM–soybean meal.

Rotifers after 2 hours of enrichment with Spirulina or ground, blended, and sieved soybean meal.

<italic>Ghrelin</italic> expression in the brain and gut.

The expression of ghrelin presented as number of ghrelin transcript copies per ng of total RNA in zebrafish brain and gut at 3-hours (B) and 24-hours (A) after feeding. The fold change presents the difference in ghrelin transcript copies between 3 and 24-hour samples. Different letters indicate statistical difference at p<0.05.

<italic>CCK</italic> expression in the brain and gut.

The expression of CCK presented as number of CCK transcript copies per ng of total RNA in zebrafish brain and gut at 3-hours (B) and 24-hours (A) after feeding. The fold change presents the difference in CCK transcript copies between 3 and 24-hour samples. Different letters indicate statistical difference at p <0.05.

<italic>Leptin</italic> expression in the brain and gut.

The expression of leptin presented as number of leptin transcript copies per ng of total RNA in zebrafish brain and gut at 3-hours (B) and 24-hours (A) after feeding. The fold change presents the difference in leptin transcript copies between 3 and 24-hour samples. Different letters indicate statistical difference at p <0.05.

<italic>Orexin</italic> expression in the brain and gut.

The expression of orexin presented as number of orexin transcript copies per ng of total RNA in zebrafish brain and gut at 3-hours (B) and 24-hours (A) after feeding. The fold change presents the difference in orexin transcript copies between 3 and 24-hour samples. Different letters indicate statistical difference at p <0.05.

<italic>NPY</italic> expression in the brain.

The expression of NPY presented as number of NPY transcript copies per ng RNA in zebrafish brain 3-hours (B) and 24-hours (A) after feeding. The fold change presents the difference in NPY transcript copies between 3 and 24-hour samples. Different letters indicate statistical difference at p <0.05.

Acknowledgments
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