Total activity, i.e., sum of all frequencies, in the shelter (A), novel tank diving (B) and scototaxis (C) tests in male and female adult offspring of wild-caught (wild), AB and fgfr1a^−/− zebrafish. Values represent individual fish and the line marks the group median. *p < 0.05, **p < 0.01, ***p < 0.001 (Mann–Whitney U-test).

Percentage duration of time spent in the open and sheltered area, respectively, in the shelter test in male and female adult offspring of wild-caught (wild, A), AB (B) and fgfr1a^−/−(C) zebrafish. Values represent group mean in the respective zone. **p < 0.01 for significant differences in time spent in open and shelter zones within the respective group (Mann–Whitney U-test).

The 14-min trial in the shelter test divided into seven 2-min time bins for total activity, i.e., sum of all frequencies (A), total distance traveled (B) and mean velocity (C) in the arena in male and female adult offspring of wild-caught (wild), AB and fgfr1a^−/− zebrafish. Data are presented as median and quartile range. No difference between the first and the last time bin was revealed within the respective groups (Wilcoxon signed-rank test).

Percentage duration of time spent in the bottom, middle and top zone, respectively, in the novel tank diving test in male and female adult offspring of wild-caught (wild, A), AB (B) and fgfr1a^−/−(C) zebrafish. Values represent group mean in the respective zone. The white lines represent the zones that are being compared in the statistical analysis. **p < 0.01, ***p < 0.001 for significant differences in time spent in the different zones within the respective group (Mann–Whitney U-test).

The 6-min trial in the novel tank diving test divided into three 2-min time bins for total activity, i.e., sum of all frequencies (A), total distance traveled (B) and mean velocity (C) in the arena in male and female adult offspring of wild-caught (wild), AB and fgfr1a^−/− zebrafish. Data are presented as median and quartile range. ^#p < 0.05 compared to the first time bin within the respective group (Wilcoxon signed-rank test).

Percentage duration of time spent in the white and black compartment, respectively, in the scototaxis test in male and female adult offspring of wild-caught (wild, A), AB (B) and fgfr1a^−/−(C) zebrafish. Values represent group mean in the respective zone. ^#p < 0.05 for significant differences in time spent in the different zones within the respective group (Mann–Whitney U-test).

The 14-min trial in the scototaxis test divided into seven 2-min time bins for total activity, i.e., sum of all frequencies (A), total distance traveled (B) and mean velocity (C) in the arena in male and female adult offspring of wild-caught (wild), AB and fgfr1a^−/− zebrafish. Data are presented as median and quartile range. ^#p < 0.05 compared to the first time bin within the respective group (Wilcoxon signed-rank test).

Scatter plot of variable loadings from the PLS-DA (first significant component R²X = 0.324, R²Y = 0.154, Q² = 0.136, second non-significant component R²X = 0.102, R²Y = 0.0716, Q² = 0.011) combining the descriptive parameters from the shelter test (ST), novel tank diving test (DT) and scototaxis test (SC) in male (M) and female (F) adult offspring of wild-caught (wild), AB and fgfr1a^−/− zebrafish. Abbreviations: D, duration (s); D/F, duration per visit (s); Dist, total distance (cm); DT, novel tank diving test; F, frequency; L, latency (s); SC, scototaxis test; ST, shelter test; Veloc, mean velocity (cm/s).