Workflow of Regeneration Rosetta app. Workflow for investigating temporal patterning of regeneration-associated genes classified within specific biological processes and/or comparative evolutionary analysis of the conserved mechanism among regenerative species, using the Regeneration Rosetta app.

Regeneration Rosetta app identifies process-specific analysis after optic nerve injury. (A) Temporal transcript profiles of genes in the cholesterol metabolic pathway. Relative transcript counts from retinas dissected 2-, 4-, 7- and 12-days post injury (dpi) were compared with those from uninjured animals (0 dpi). Transcript expression is presented as TPM Z-scores; putative SREBF2 target genes are indicated to the right of the heatmap (biosynthetic enzymes in black; transcription factors are in red). (B) Specific enrichment of cholesterol metabolic and biosynthetic genes early in regeneration. Fisher’s exact test of over-representation was used to identify cholesterol-related GO-terms correlated with specific stages of regeneration.

Regeneration Rosetta app identifies conserved core regulators of CNS axon regeneration. Venn diagram of axon growth-associated genes from regenerating CNS neurons after zebrafish optic nerve injury (ONI; retina response) and lamprey spinal cord injury (SCI; spinal cord (SC) and brain responses). Approximately 10–20% of regeneration-associated genes are shared between neurons regenerating axons in brain, spinal cord and optic nerve, including a core set of 21 regeneration-associated transcription factor encoding genes that are homologous to human genes (listed in the middle with HGNC gene symbol).