FIGURE SUMMARY
Title

Generation of a Triple-Transgenic Zebrafish Line for Assessment of Developmental Neurotoxicity during Neuronal Differentiation

Authors
Koiwa, J., Shiromizu, T., Adachi, Y., Ikejiri, M., Nakatani, K., Tanaka, T., Nishimura, Y.
Source
Full text @ Pharmaceuticals (Basel)
Figure 1

Schematic representation of transposon vectors.
Figure 2

In vivo fluorescence imaging of the triple-Tg zebrafish line.
Figure 3

Bright-field images of triple-Tg zebrafish exposed to the maximum tolerable concentrations of chemicals during early development. (A) Experimental protocol. (B) Triple-Tg zebrafish were treated with the indicated chemicals at their maximum tolerable concentrations from 12 hpf to 5 dpf. The animals were then anesthetized and subjected to in vivo bright-field imaging using a stereomicroscope.
Figure 4

Quantification of in vivo fluorescence imaging of triple-Tg zebrafish exposed to chemicals at the maximum tolerable concentrations during early development. Triple-Tg zebrafish were treated as described for Figure 3 and subjected to in vivo fluorescence imaging at 5 dpf. The fluorescence signals for CFP (A), RFP (B), YFP (C), RFP/CFP ratio (D), YFP/CFP ratio (E), and YFP/RFP ratio (F) were quantified and normalized to the mean signals in the untreated control zebrafish group (CNT). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data are presented as the mean ± SEM of 4–77 zebrafish/chemical.
Figure 5

Hierarchical clustering of chemicals based on their effects on Cerulean (CFP), mCherry (RFP), and mCitrine (YFP) expression in triple-Tg zebrafish. The normalized score of six fluorescence parameters (CFP, RFP, YFP, RFP/CFP, YFP/CFP, and YFP/RFP) from triple-Tg zebrafish exposed to chemicals at their MTC from 12 hpf to 5 dpf were subjected to hierarchical clustering using Manhattan distance with average linkage.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Pharmaceuticals (Basel)
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