Effect of NG on the number of thrombocytes in zebrafish induced by vinorelbine (NVB). The number of CD41-GFP⁺ cells was counted in the caudal hematopoietic tissue area of zebrafish. (a) Phenotypes of larvae of Tg (CD41 : EGFP) lines. (b) Number of CD41-GFP⁺ cells at 72 hpf. The values are expressed as mean ± SEM (n = 10). ^∗∗∗∗P < 0.001 versus Ctl, ^∗∗P < 0.01, ^∗∗∗P < 0.001 versus NVB.

Effect of NG on the number of thrombocytes in zebrafish induced by chloramphenicol (CHL). The number of CD41-GFP⁺ cells was counted in the caudal hematopoietic tissue area of zebrafish. (a) Phenotypes of larvae of Tg (CD41 : EGFP) lines. (b) Number of CD41-GFP⁺ cells at 72 hpf. The values are expressed as mean ± SEM (n = 10). ^∗∗∗∗P < 0.001 versus Ctl, ^∗∗P < 0.01, ^∗∗∗P < 0.001 versus CHL.

Effect of NG on the number of erythrocytes in zebrafish induced by methotrexate (MTX). (a) Phenotypes of larvae of wild-type AB lines. (b) The staining intensity of erythrocytes at 48 hpf. The values are expressed as mean ± SEM (n = 30). ^∗∗∗∗P < 0.001 versus Ctl, ^∗P < 0.05, ^∗∗P < 0.01 versus MTX.

Effect of NG on the number of erythrocytes in zebrafish induced by doxorubicin (ADM). (a) Phenotypes of larvae of wild-type AB lines. (b) The staining intensity of erythrocytes at 48 hpf. The values are expressed as mean ± SEM (n = 30). ^∗∗P < 0.01 versus Ctl, ^∗P < 0.05, ^∗∗P < 0.01 versus ADM.

Effect of NG on the number of erythrocytes in zebrafish induced by mechlorethamine hydrochloride (MH). (a) Phenotypes of larvae of wild-type AB lines. (b) The staining intensity of erythrocytes at 48 hpf. The values are expressed as mean ± SEM (n = 30). ^∗∗∗∗P < 0.0001 versus Ctl, ^∗P < 0.05, ^∗∗P < 0.01 versus MH.

Expression of related genes in zebrafish hematopoietic injury model pretreated by methotrexate (MTX). The mRNA levels of scl1 (a), c-myb (b), GATA1 (c), pu.1 (d), and Runx1 (e). The values are expressed as mean ± SEM (n = 30). ^∗P < 0.05, ^∗∗P < 0.01 versus Ctl. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 versus MTX.