Dose-related effect of sulfolane exposure in µg/mL on the yolk sac utilisation ( A), larval length ( B) and eye volume ( C) quantified in zebrafish (Danio rerio) larvae at 72 and 120 hpf. Yolk sac width was converted to percent utilisation by establishing the width at 120 hpf for control (0 µg/mL) zebrafish larvae as 100% yolk sac utilisation. Larval length was measured from the tip of the head to the tip of the tail. The horizontal and vertical eye diameters were averaged and converted to eye volume based on the assumption that the eye is a sphere[ 35]. The points represent mean and SEM. Unmatched letters represent statistical significance (p < 0.05) based on tests with Conover?Iman all pairs comparison test. n = 36?40 individual larvae.

Dose-related effect of sulfolane exposure in µg/mL on the presence of yolk sac ( A), pericardial oedemas ( B), haemorrhage ( C), spinal malformation ( D), and uninflated swim bladder ( E) in zebrafish (Danio rerio) larvae at 120 hpf. The points represent mean and SEM. Unmatched letters represent statistical significance (p < 0.05) based on tests with Conover?Iman all pairs comparison test. n = 8?15 wells, each containing 10 embryos

Dose-related effect of sulfolane exposure in µg/mL on the response to touch stimuli in zebrafish (Danio rerio) larvae at 120 hpf. The points represent mean and SEM. Unmatched letters represent statistical significance (p < 0.05) based on tests with Conover?Iman all pairs comparison test. n = 8?15 wells, each containing 10 embryos.

Dose-related effect of sulfolane exposure in ?g/mL on the presence of various characterized

morphometric endpoints in zebrafish (Danio rerio) larvae at 72hpf. The points represent mean and SEM.

Unmatched letters represent statistical significance (p < 0.05) based on results of Conover-Iman all pairs

comparison test. n = 6?10 wells, each containing 10 embryos. (A) Yolk Sac Oedema (B) Pericardial Oedema

(C) Hemorrhaging (D) Spinal Malformations.