- Title
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ORC-13661 protects sensory hair cells from aminoglycoside and cisplatin ototoxicity
- Authors
- Kitcher, S.R., Kirkwood, N.K., Camci, E.D., Wu, P., Gibson, R.M., Redila, V.A., Simon, J.A., Rubel, E.W., Raible, D.W., Richardson, G.P., Kros, C.J.
- Source
- Full text @ JCI Insight
ORC-13661 blocks entry of neomycin-Texas Red into brn3c:gfp zebrafish lateral line hair cell bodies in vivo. Time-lapse imaging of lateral line neuromasts of brn3c:gfp-transgenic zebrafish exposed to neomycin-Texas Red (neo-TR) with and without ORC-13661. Images were captured at 30-second intervals for 50 minutes immediately following neo-TR treatment at a concentration of 200 μM. In the top row of images, hair cells show a progressive accumulation of intracellular neo-TR (200 μM). Some cells are dying by 20 minutes. In the bottom row of images, hair cells are exposed to neo-TR (200 μM) and cotreatment with ORC-13661 (8.33 μM). At this concentration, cotreatment with ORC-13661 virtually eliminates red fluorescence in hair cell bodies and all cells survive. Times at the top of each panel indicate time (minutes) from introduction of neo-TR. Scale bar: 1 μm. |