Paclitaxel (PTX) Inhibition on Tumor Growth in Zebrafish Tumor-Xenograft Model

(A) Fluorescent images of zebrafish tumors treated with or without PTX. The siRNA-transfected cells were pre-stained with PKH26 red fluorescent dye for clear observation. Labeled cells were injected into zebrafish using a single injection with ∼850 cells per embryo. After zebrafish were treated with or without PTX dissolved in aerated water containing 1× phenylthiourea for various days (0–2 days), the fluorescent images of tumor sites were observed using fluorescent microscopy. (B) Tumor growth inhibition is presented in the bar graphs. Mean fluorescent areas of tumor sites in zebrafish were analyzed using ImageJ software, and statistical analysis was performed using the two-tailed Student’s t test (n = 30; *p < 0.05, **p < 0.01).