Evaluation of rh_thrombin, rh_sc_thrombin and [rh_sc_thrombin][ox890] hMSC plasma membrane affinity. Cells labelled with CellMask™ Deep Red (green) and corresponding rhodamine labelled thrombin (magenta). a Native (rh_thrombin) thrombin at T = 0 h. b Cationised (sc_rh_thrombin) thrombin at T = 0 h. c Polymer surfactant conjugate ([rh_sc_thrombin][ox890]) thrombin at T = 0 h. Scale bars represent 50 µm. d MTS cell metabolic activity assay (n = 8) of hMSCs after labelling with [sc_thrombin][ox890] compared to unlabelled controls. Data reported as means ± s.d. Dunnett’s test; *p ≤ 0.05, **p ≤ 0.001. Source data are provided as a Source Data file

In vivo zebrafish injury and [sc_thrombin][ox890] labelled GFP + fibroblast addition. Schematic representation of the in vivo adult zebrafish injury model. a Wildtype (non-transgenic) recipient zebrafish were anaesthetized and a 4 mm incisional injury made on the ventral upper thorax. A lateral view is shown. b Unlabelled or [sc_thrombin][ox890] labelled, FACS sorted GFP+ fibroblasts were injected at six sites around the edge of the incisional injury. At the desired time-point, fish were sacrificed and the tissue surrounding the incision was fixed, imaged and embedded for sectioning. A ventral view is shown. Ventral view of the area of tissue surrounding the incision at 3 dpi following transfer of c unlabelled or d [sc_thrombin][ox890] labelled GFP+ fibroblasts. Similar numbers of cells were retained at all wounds. The red line depicts the approximate position of the incisional injury which is fully re-epithelialized at this stage. Sections through the injury region at 3 dpi following transfer of e unlabelled or f [sc_thrombin][ox890] labelled GFP+ fibroblasts. No obvious differences were observed between wounds containing labelled or unlabelled cells. Arrows indicate the position of the incision. All scale bars represent 250 µm