Kinematic analysis of the swimming showing ataxic gait in acrylamide (ACR)-exposed zebrafish. (A) For each frame, the kinematic analysis software divides the fish body in three segments of the same length, measuring the angles α, β and γ. (B) Time-course of angles α, β and γ from a representative control zebrafish (time in milliseconds). (C) Representative kinematic traces (angle γ) of control and ACR-treated fish. (D) Average curvature measured by angles α, β and γ over half a cycle of bending for control and ACR-exposed fish (mean ± SE; n = 7 for control and n = 6 for ACR-exposed fish). (E) Average tail-beat amplitude for control and ACR-exposed fish (mean ± SE; n = 7 for control and n = 6 for ACR-exposed fish). **p < 0.01 Student’s t-test.