- Title
-
SOX9 drives the epithelial-mesenchymal transition in non-small-cell lung cancer through the Wnt/β-catenin pathway
- Authors
- Huang, J.Q., Wei, F.K., Xu, X.L., Ye, S.X., Song, J.W., Ding, P.K., Zhu, J., Li, H.F., Luo, X.P., Gong, H., Su, L., Yang, L., Gong, L.Y.
- Source
- Full text @ J Transl Med
SOX9 induces EMT and promotes distant metastasis in NSCLC cells and a zebrafish model of NSCLC. a, b Western blotting and immunofluorescence analyses were used to determine the protein level of epithelial cell markers (E-cadherin and γ-catenin) and mesenchymal cell markers (vimentin and N-cadherin) in A549-SOX9 and NCI-H460-SOX9 cell lines and their corresponding control A549-Vector and NCI-H460-Vector cells. c, d Western blotting and immunofluorescence analyses were used to determine the protein level of epithelial cell markers (E-cadherin and γ-catenin) and mesenchymal cell markers (vimentin and N-cadherin) in A549-SOX9 sh1# and NCI-H460-SOX9 sh1# cells and their corresponding control A549-pSuper-Vector and NCI-H460-pSuper-Vector cells. e, f Zebrafish embryos injected with either SOX9-overexpression cells or SOX9-knockdown cells were imaged live under an Olympus® BX51 microscope. SOX9-overexpressing cells and SOX9-knockdown cells are labeled with red fluorescence and zebrafish are labeled with green fluorescence. Quantification of the number of disseminated tumor foci (n = 5 per group) in the tail regions of Zebrafish embryos, ***p < 0.0001 |