- Title
-
α-Melanocyte-Stimulating Hormone Attenuates Neovascularization by Inducing Nitric Oxide Deficiency via MC-Rs/PKA/NF-κB Signaling
- Authors
- Weng, W.T., Wu, C.S., Wang, F.S., Wu, C.Y., Ma, Y.L., Chan, H.H., Wu, D.C., Wu, J.C., Chu, T.H., Huang, S.C., Tai, M.H.
- Source
- Full text @ Int. J. Mol. Sci.
Effect of l-Arginine on vascular development and endothelial recruitment in α-MSH-treated transgenic zebrafish. (A) Effect of l-Arg supply (5 mM) on vascular development and endothelial recruitment in α-MSH-treated transgenic zebrafish. Representative confocal images of phenotype and vascular growth of ISV in Tg(kdrl:mCherryci5;fli1a:negfpy5) double transgenic zebrafish embryos. The l-Arg-treated embryos displayed minor responses to the α-MSH inhibition of vessel formation (red fluorescence) and endothelial cell recruitment (green fluorescence in cell nuclei). Solid and hollow arrowheads indicate complete and incomplete vessel formation in ISV. (B) Quantification analysis of the ISV completion rate in ISV of α-MSH-treated transgenic zebrafish. (C) Quantification analysis of the number of endothelial cells per ISV of α-MSH-treated transgenic zebrafish. Data are expressed as mean ± SEM from 30 ISVs in at least 3 embryos. Asterisks indicated statistical significance versus control (** p < 0.001); Scale bars in the upper, middle, and lower panels were 50 μm, 50 μm and 100 μm, respectively. |