Study on motility of cells in a zebrafish model. (A) The number of DII labelled cell injected to perivitelline cavity of 48 hpf zebrafish embryos were similar for PC3 (the left panel), PC3-S18-2-CL03 (the middle panel) and PC3-S18-2-CL04 (the right panel) cells. (B) The number of cells migrated to the tail of 120 hpf zebrafish embryos (after 3 days of injection). The arrows indicate the cells migrated to the tail. Notice the larger number of migrated PC3-S18-2-CL04 cells, in comparison with PC3-S18-2-CL03 and parental PC3 cells. Statistical analysis was performed, using GraphPrism 6 software. (C) Migration of PC3-S18-2-CL04 to tail of zebrafish after down regulation of S18-2 using siRNA (the right panel). Embryos were also injected after treatment with control siRNA (the right panel). The graph shows significant difference after down regulation of S18-2. (D) After DII labelling cells were incubated overnight with α-CXCR4 (the right panel) or isotype control antibody (the left panel). Cells were injected into embryos along with CXCL12. Three days after injection cells were counted in both the groups. The graphical representation shows significant difference of migrated cells among the two groups. At least 20 embryos were injected every time, and experiments were performed minimum 3 times.