Fig. 5

Diagram of the OPT rotational acquisition procedure for zebrafish. For each projection angle θ, the focal plane is scanned through the entire zebrafish to create a full projection, even with a shallow depth-of-field objective.

Fig. 6

We used focal plane scanning OPT (FPS-OPT) to image the head of a Tg(fli1a:eGFP) zebrafish in 3D fluorescence with a 10 ×/0.3 NA air objective. Under such conditions, single-plane OPT would be unable to produce an acceptable reconstruction due to the large sample thickness and shallow depth-of-field. With FPS-OPT, we compare 3D reconstructions from (a) standard filtered backprojection (FBP) and (b) our proposed PSF-aware FBP. Our proposed PSF-aware FBP algorithm reconstructs an image with less out-of-focus blur. Scalebar is 100 μm.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomed. Opt. Express
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