- Title
-
Anti-Infective Effect of Adhesive Probiotic Lactobacillus in Fish is Correlated With Their Spatial Distribution in the Intestinal Tissue
- Authors
- He, S., Ran, C., Qin, C., Li, S., Zhang, H., de Vos, W.M., Ringų, E., Zhou, Z.
- Source
- Full text @ Sci. Rep.
Histology (H&E staining) of intestine of zebrafish after immersion treatment with Lactobacillus strains for 14 days. CON, control; LGG, L. rhamnosus GG; PB22, LGG mutant PB22; LR, L. rhamnosus JCM 20300 Asterisk, edema and floating of the intestinal mucous membrane. Arrow, degeneration and focal necrosis of intestinal villus. |
Confirmation of mucus separation by Alcian blue staining. (A) The intestinal mucus stained with Alcian blue (B) The intestine mucus after 90s vortexing treatment. (C) The intestine mucus after 180 s vortexing treatment. After vortexing at 250 rev min?1 for 180s, there is no mucus left in the intestine. |