FIGURE SUMMARY
Title

Green fluorescent genetically encoded calcium indicator based on calmodulin/M13-peptide from fungi

Authors
Barykina, N.V., Subach, O.M., Piatkevich, K.D., Jung, E.E., Malyshev, A.Y., Smirnov, I.V., Bogorodskiy, A.O., Borshchevskiy, V.I., Varizhuk, A.M., Pozmogova, G.E., Boyden, E.S., Anokhin, K.V., Enikolopov, G.N., Subach, F.V.
Source
Full text @ PLoS One
Fig. 6

Calcium imaging of neurons expressing FGCaMP in zebrafish larva at 4 days post fertilization.

(A) Overlay of confocal fluorescence images of neurons expressing FGCaMP, acquired with 488 and 405 nm excitation and 525/50BP emission. White box indicates area zoomed-in in panel B. Scale bar, 100 μm. FB, forebrain; MB, midbrain; HB, hindbrain. (B) High magnification images of the neurons highlighted in the white box in panel A acquired with 488 nm excitation and 525/50BP emission (left; green pseudocolor) and 405 nm excitation and 525/50BP emission (middle; blue pseudocolor), and overlay of the left and middle images (right). Scale bar, 50 μm. (C) Representative single cell recording of GCaMP6f (top; magenta) and FGCaMP green fluorescence responses (bottom; green) during 4-aminopyridine induced neuronal activity (Ex: 475/34BP, Em: 527/50BP). Population data for (D) maximum fluorescence changes ΔF/F and (E) SNR corresponding to the experiment in panel C (26 neurons in 3 fish and 43 neurons from 5 fish for GCaMP6f and FGCaMP, respectively). Box plots with notches are used (narrow part of notch, median; top and bottom of the notch, 95% confidence interval for the median; top and bottom horizontal lines, 25% and 75% percentiles for the data; whiskers extend to 5th and 95th percentile for the data; horizontal bar is mean). (F) Representative single cell recording of FGCaMP green fluorescence excited with 488 and 405 nm laser illumination during 4-aminopyridine induced neuronal activity. (G) Fluorescence ratio for the traces shown in panel F. (H) Population data for fluorescence ratio for experiment in panel C (107 neurons in 2 fish). Box plots with notches are used (see panel D for description).
Fig. N

Confocal ratiometric calcium imaging with FGCaMP during 4-AP induced neuronal activity in paralyzed larvae embedded in ultra-low-melting agarose gel. Example of frame from S1 Video is shown.
Fig. O

Light sheet ratiometric calcium imaging with FGCaMP during 4-AP induced neuronal activity in paralyzed larvae embedded in ultra-low-melting agarose gel. Example of frame from S2 Video is shown.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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